维生素D3羟化酶生产菌的原生质体诱变育种  

Breeding of vitamin D_3 hydroxylase producing strain byprotoplast mutagenesis with ultraviolet irradiation

作　　者：苏宏飞[1] 黄飞 韦春葵[1] 熊思驰[1] 李丽[1] 王国盼[1] 黄时海[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]南宁高新区,广西南宁530007

出　　处：《中国酿造》2013年第1期49-52,共4页China Brewing

基　　金：南宁市科学研究与技术开发计划项目(20125193);梧州市科学研究技术开发计划项目(201201023)

摘　　要：原始菌株放线菌CXX-10具有维生素D325位羟化活性。通过CXX-10原生质体的制备和再生条件优化,并进行紫外诱变,获得一株酶活较原始菌株提高到152%的菌株UV-179,25(OH)D3产量达到4.27mg/L。Actinomycete CXX-10 has the ability of 25-hydroxylating vitamin D3. In this work, a mutant （UV-179） containing high hydroxylation activity was obtained by protoplast mutagenesis of ultraviolet irradiation after protoplast preparation and regeneration condition optimization. The production of 25（OH）D3 reached 4.25mg/L, which was 1.52 fold of production of the start strain.

关 键 词：25-位羟化 原生质体 紫外诱变 

分 类 号：Q93-331[生物学—微生物学]