酸菜包外包膜污染菌分析  

Analysis of polluted microorganisms on the outer packages of Chinese sauerkraut

作　　者：蒋荣荣[1] 赵炜[2] 王洋[1] 周帼萍[1] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]南通出入境检验检疫局,江苏南通226002

出　　处：《中国酿造》2013年第1期133-136,共4页China Brewing

基　　金：湖北省教育厅科学研究计划项目中青年项目(Q20111703);武汉工业学院研究生创新基金项目(2011cx002)

摘　　要：某企业发现直接接触食品的酸菜外包膜微生物超标。送检的5份细菌总数达104cfu/mL^107cfu/mL,并伴有霉菌污染。通过16S rDNA序列分析,鉴定其主要的污染菌为微小杆菌(Exiguobacterium spp.)和不动杆菌(Acinetobacter spp.),同时也伴有部分葡萄球菌(Staphylococcus spp.)和芽孢杆菌(Bacillus spp.)。污染的霉菌则主要为曲霉(Aspergillus spp.)、交链孢霉(Alternaria spp.)和青霉(Penicillium spp.)。采用半定量粘附实验检测其生物膜形成能力,发现3个主要污染菌株均有很强的生物膜形成能力。It was found that the microbial counts of some outer packages of Chinese sauerkraut which will directly contact with foods were out of specification. 104cuf/ml-107cuf/ml bacterial counts were found in the samples, some also with fungi. The main contaminants identified by 16S rDNA sequence analysis were Exiguobacterium spp.and Acinetobacter spp. with some Staphylococcus spp. and Bacillus spp. While the main fungi detected were Aspergillus spp., Alternaria spp., and Penicillium spp. With semi-quantitative adhesion assay, the three isolates were found that it have strong biofilm forming capability.

关 键 词：外包膜 污染菌 微小杆菌 不动杆菌 生物膜 

分 类 号：Q939.97[生物学—微生物学]