血红素加氧酶-1在门静脉高压性胃病患者胃黏膜的表达  

Expression of heme oxygenase-1 in gastric mucosa of patient with portal hypertensive gastropathy

作　　者：白云[1] 姜慧卿[1] 路新卿[1] 张晓岚[1] 任锡玲[1] 马俊骥[1] 

机构地区：[1]河北医科大学第二医院消化内科河北省消化病研究所河北省消化病实验室,石家庄050000

出　　处：《中华消化内镜杂志》2013年第2期75-78,共4页Chinese Journal of Digestive Endoscopy

基　　金：河北省自然科学基金资助项目（0303483）

摘　　要：目的探讨血红素加氧酶-1（HO-1）在肝硬化门静脉高压性胃病（PHG）患者胃黏膜的表达情况及其意义。方法分别采集22例正常受试者（对照组）、20例门静脉高压（PHT）患者（PHT组）和22例PHG患者（PHG组）的胃黏膜标本，观察胃黏膜组织学变化，测定门静脉血流量（PVF）。应用免疫组织化学法和Western blot方法检测HO-1蛋白在胃黏膜组织的表达情况，并分析HO-1蛋白与PVF、内镜下PHG严重程度的相关性，以及内镜下PHG严重程度与临床参数的相关性。结果PHG组和PHT组患者胃黏膜中HO-1蛋白表达明显高于对照组（P〈0．05），而PHG组与PHT组之间比较差异无统计学意义（P〉0．05）。PHG患者胃组织HO-1蛋白表达与内镜下PHG严重程度积分呈显著正相关（r=0．459，P〈0．05）。内镜下PHG严重程度积分与食管静脉曲张程度（r=0．059，P〉0．05）、Child—Pugh分级（r=-0．001，P〉0．05）均无显著性相关。PHG组与PHT组患者的胃黏膜组织HO-1蛋白表达与PVF无显著性相关（r=0．071，P〉0．05）。结论HO-1蛋白在PHG患者胃黏膜中呈高表达，参与了PHG患者胃黏膜血液循环紊乱的发生。Objective To explore the expression of heme oxygenase-1 （ HO-1 ） in gastric mucosa of patient with portal hypertensive gastropathy （ PHG）, and its role in the pathogenesis of PHG. Methods The specimens were obtained from the gastric mueosa of 22 healthy subjects, 20 portal hypertension （PHT） without PHG, and 22 PHG patients. Histological changes of the gastric mucosa were detected, and portal ve- nous flow （PVF） was measured. The expression of HO-1 protein in gastric mucosal specimens was assessed by immunohistochemistry and Western blot analysis, respectively. The relationship between HO-1 expression and PHG severity score, HO-1 expression and PVF, PHG severity score and clinical parameters were investi- gated. Results HO-1 protein expression in gastric mucosa of PHG and PHT was significantly higher than that of the control （ P 〈 0.05 ） , while there was no significant difference in this variable between PHG and PHT （P 〉 0. 05 ）. The positive correlation was detected between HO-1 expression and PHG severity score in PHG patients （ r = 0. 459, P 〈 0. 05 ） , however, PHG severity score was irrelevant to severity of esophageal varice （ r = 0. 059, P 〉 0.05 ） or to Child-Pugh classification （ r = - 0. 001, P 〉 0. 05 ）. Of all the patients with PHT and PHG, no significant correlation was found between HO-1 expression and PVF （r = 0. 071, P 〉 0. 05）. Conclusion HO-1 protein is up-regulated in gastric mucosa of PHG patients, which may con- tribute to gastric circulation disorder of PHG.

关 键 词：血红素加氧酶-1 一氧化碳 门静脉高压性胃病 

分 类 号：R575.2[医药卫生—消化系统]