负压吸引式小肠镜进行小肠检查的可行性实验研究  

The feasibility of negative pressure suction enteroscopy for small intestine examination

作　　者：刘金华[1] 张忠鲁[2] 王利[1] 刘丹阳[3] 孔繁红[4] 刘领君[1] 

机构地区：[1]辽宁省大连市友谊医院胃肠外科,116001 [2]辽宁省大连市友谊医院院办,116001 [3]辽宁省大连市友谊医院内分泌科,116001 [4]大连市中心医院

出　　处：《中华消化内镜杂志》2013年第2期94-96,共3页Chinese Journal of Digestive Endoscopy

基　　金：大连市卫生局课题（DLSWSJ2008--004）

摘　　要：目的评价负压吸引式小肠镜用于小肠检查的可行性。方法对新鲜的带完整肠系膜的猪小肠20根进行负压吸引式小肠镜检查，探讨适合的工作压强，并观察镜检后肠道损伤情况；根据离体实验结果指导活猪小肠检查，观察镜检情况、肠壁情况及术后2周随访的不良反应发生情况。结果负压吸引式小肠镜检查离体猪小肠的起始工作压强绝对值要大于0．06MPa，在此基础上20根猪小肠均顺利完成检查，平均耗时（40±20）min，负压吸引过的肠壁行组织学切片观察未发现肠壁组织受损。以绝对值略高于0．06MPa作为起始工作压强进行活体猪实验时检查过程中出现了局部肠道滑动现象，当压强绝对值大于1．00MPa后不再出现局部肠道滑动现象，在此基础上20只活猪均顺利完成小肠检查，平均耗时（60±20）min，肠道的不同抓吸点的组织结构完整，切片镜下见小肠绒毛结构清晰，黏膜下层及肌层完整。检查结束解除麻醉后均能进食，其中18只术后第2天排便，另外2只第3天排便，术后2周的观察期内20只猪均未出现腹胀、便血、便秘等情况，饮食、活动、睡眠及二便均正常。结论负压吸引式小肠镜用于小肠检查是可行的，具有较好的研发前景。Objective To evaluate the feasibility of negative pressure suction enteroscopy for intesti- nal examination. Methods The negative pressure suction enteroseopy was first applied to examine 20 de- tached small intestines from pigs to explore a suitable work pressure and to detect possible intestinal dama- ges. The procedure was then applied in living pigs to evaluate the feasibility and to observe possible adverse reactions in a 2-week follow-up. Results The absolute working pressure of the negative pressure suction en- teroseopy should be higher than 0. 06 Mpa. The procedures were successfully completed in all 20 pig small in- testines in 40 ± 20 min. There was no tissue damage in the intestinal wall according to histological findings. Local glide occurred in living pig examination at 0.06 Mpa, and it disappeared when the absolute value was higher than 1.00 Mpa. The procedure was completed in 20 living pigs in 60± 20 min. Histological findings showed complete tissue structure, clear villi and complete submucous and muscular tissue. All pigs could take food at recovery from anaesthesia. 18 had bowel movement on the 2nd day after the operation, and the 2 others recovered on the 3rd day. No abdominal distension, hematochezia, or constipation was observed in 2-week fol- low-up. Food intake, activity, sleep, urination and defecation were all normal. Conclusion The negative pressure suction enteroscopy is effective and safe for small intestine examination, and is of good prospect.

关 键 词：动物实验 内窥镜检查 小肠 负压吸引式小肠镜 

分 类 号：R574[医药卫生—消化系统]