五鹤续断对阿尔茨海默病大鼠顶叶和海马β淀粉样蛋白表达的影响  

Effect of dipsacus on the expression of β-amyloid in parietal and hippocampus of Alzheimer＇s disease rats

作　　者：谭文波[1] 袁德培[1] 杨付明[1] 

机构地区：[1]湖北民族学院医学院,恩施445000

出　　处：《中华老年医学杂志》2013年第2期212-214,共3页Chinese Journal of Geriatrics

基　　金：国家中医药管理局公共卫生专项资金项目（财社[2010]91号）

摘　　要：目的研究五鹤续断抗阿尔茨海默病（AD）的作用。方法将40只大鼠随机分为空白对照组、模型组、五鹤续断组和维生素E组，根据实验要求给药处理42d，同时观察各组大鼠一般情况，Morris水迷宫实验检测大鼠学习记忆能力，采用双抗体夹心法测定各组大鼠顶叶皮质和海马β淀粉样蛋白（Ap）的含量。结果模型组与对照组比较一般情况明显恶化逃避潜伏期均明显延长（P〈0．01），原平台象限活动时间明显减少（t=4．6261，P：0．0002），穿越原平台次数明显减少（t=6．5335，P=0．0000），大鼠顶叶皮质和海马AB含量显著增加（t=5．2499，P=0．0001）。五鹤续断组、维生素E组分别与模型组比较：一般情况均明显改善；逃避潜伏期均明显缩短（P〈0．01）；原平台象限活动时间明显增加，分别为（45．76±12．15）s、（48．70±10．25）s和（30．20±10．48）s（t=3．0666、3．9908，p=0．0066、0．0009）；穿越原平台次数明显增多，分别为（3．02±1．19）次／2rain、（3．56±0．85）次／2rain和（1．43±1．24）次／2rain（t=2．9256、4．4804，P=0．0090、0．0003）；大鼠顶叶皮质和海马A口含量显著减少，顶叶皮质分别为（280．37±51．40）Pg／g、（263．14±45．52）pg／g和（337．46±70．51）pg／g（f=2．1164、2．8003，P=0．01 85、0．01]8），海马分别为（295．60±67．58）pg／g、（274．38±57．56）Pg／g和（388．26±83．72）pg／g（t=2．9256、4．4804，P=0．0090、0．0003）。结论五鹤续断可显著降低智能相关脑区Ap的表达，具有抗AD作用，其作用的活性成分可能是其所含天然维生素E。Objective To study the effect of dipsacus on the expression of 13 amyloid in parietal cortex and hippocampus of Alzbeimer＇s disease model rats. Methods A total of 40 rats were randomly divided into control group, model group, dipsacus group and vitamin E group （n= 10, each） General situation of rats was observed after 42 days of dipsacus treatment. The ability of learning and memory in rats was tested by Morris water maze. The content of βamyloid in parietal cortex and hippocampus of each group rats were measured using double antibody sandwich method. Results Model group compared with control group showed that general conditions deteriorated markedly; escaping delitescence was prolonged signifieantly（P〈~0.01） ; the activity time on the former platform quadrant reduced significantly （ t=4. 6261, P 0. 0002） ; the number of crossing the former platform reduced significantly （ t=6. 5335, P=0. 0000） ; the content of A13 in parietal cortex and hippocampus increased significantly （ t=4.2812, P：0.0004 ） （ t=5.2499, P=0.0001）. Dipsacus group and vitamin E group compared with model group showed that general condition improved significantly escape latency was shortened significantly（P〈0.01）; the activity time on the former platform quadrant increased significantly （45. 76±12.15） s, （48.70±10.25） s and （30.20!10.48） s （ t 3.0666, 3. 9908, P= 0.0066, 0.0009）]; the number of crossing the former platform increased significantly [（3.02±1.19） t/2min, （3.56±0.85） t/2min and （1.43±1.24） t/2min （ t=2.9256, 4. 4804, P = 0.0090, 0.0003）; the contenl of A13 in parietal cortex and hippocampus reducedsignificantly in parietal cortex （（280.37±51.40） pg/g, （263. 14±45.52 ） pg/g versus （337.46± 70.51） pg/g （t=2. 1164, 2. 8003, P=0. 0485, 0. 0118）1, in hippocampus ^（295.60±67.58）pg/g, （274.38!57.56）pg/g versus （388.26--83.72）pg/g（t=2. 9256, 4. 4804, P=0. 0090, 0. 0003）1. Conclusions Dipsacus can reduce expression of AI3 in brain a

关 键 词：阿尔茨海默病 顶叶 海马 淀粉样蛋白 维生素E 

分 类 号：R285.5[医药卫生—中药学]