SLP-2在胃癌组织中的表达及对胃腺癌细胞SGC7901运动侵袭的影响  

Expression of stomatin like protein-2 in gastric adenocarcinoma and its effects on the migration and invasion of SGC7901 cell

作　　者：张剑[1] 李建生[1] 吴敏[2] 赵立群[3] 张自森[2] 赵晔[1] 

机构地区：[1]郑州大学第一附属医院消化内科河南省高校临床医学重点学科开放实验室,450052 [2]郑州大学第五附属医院肿瘤科 [3]河南省(郑州大学)医药科学研究院

出　　处：《中华消化杂志》2013年第1期37-41,共5页Chinese Journal of Digestion

摘　　要：目的探讨SLP-2基因在胃腺癌组织中的表达，及其对胃腺癌细胞SGC7901运动侵袭的影响。方法采用免疫组织化学法检测SLP2在胃腺癌患者癌组织、配对癌旁组织（各60份）中的表达情况，分析其与患者临床病理特征的关系。将靶向SLP-2基因的小分子干扰RNA（siRNA）瞬时转染SGC7901细胞，采用PCR法和Western印迹法分析转染后SGC7901细胞SLP-2mRNA和蛋白表达情况，采用Transwell细胞迁移实验及Matrigel基质体外侵袭实验检测转染后SGC7901细胞运动侵袭能力的改变。统计学处理采用Z‘检验及LSD-t检验。结果SI．P-2蛋白在胃腺癌组织中的表达率为68．3％（41／60），高于癌旁组织的25．0％（15／60）（X2=22．634，P〈0．01）。SI。P-2蛋白在较晚期胃腺癌组织中的表达水平高于较早期胃腺癌组织，在有淋巴结转移病例中的表达水平高于无淋巴结转移的病例（X2=4．962、3．994，P均〈0．05），其表达水平与性别、年龄、肿瘤大小、分化程度无相关性（P均〉0．05）。SLP-2siRNA转染后，SGC7901细胞中SLP-2mRNA和蛋白的表达显著降低。SLP-2siRNA转染后48h，SGC7901细胞迁移能力[穿膜细胞数为（53．0±10．8）个]明显低于阴性对照组和空白对照组[（75．8±13，4）和（78．4±15．2）个]（t=22．778、25．444，P均〈O．01）；SGC7901细胞的体外侵袭能力[穿膜细胞数为（36．3±8．9）个]也明显低于阴性对照组和空白对照组[（53．9±12．1）和（49．8±9．O）个]（t=17．556、13．444，P均〈0．01）。结论SLP-2在胃腺癌组织中高表达，SLP-2可能通过促进胃腺癌细胞的运动侵袭能力，参与胃腺癌的转移。Objective To investigate the expression of stomatin like protein 2 （SLP-2） in gastric adenocarcinoma and its effects on migration and invasion of SGC7901 cell line. Methods The expression of SLP-2 in 60 pairs of gastric adenocarcinoma tissue specimens and corresponding adjacent tumor tissue specimens was detected by immunohistochemistry, and its relation with clinical pathological characteristics was also analyzed. SGC7901 cells were transient transfected with SLP-2 siRNA. After transfection, the expression of SLP-2 in SGC7901 cells at mRNA and protein level was detected by reverse transcripton-polynerase chain reaction （RT PCR） and Western blot. The changes of migration and invasion of SGC7901 cells after transfection were observed through Transwell test and Matrigel in vitro invasion test. The data were analyzed by X2 test and LSD-t test. Results The high expression percentage of SLP 2 in gastric adenocarcinoma tissue was 68. 3% （41/60）, which was significantly higher than that of the adjacent tumor tissues （25.0%, 15/60; X2=22. 634, P〈0.01）. The expression of SLP-2 in late stage gastric adenocareinoma tissues was higher than that of early stage gastric adenocarcinoma tissues （X2= 4. 962, P〈0. 05）. The expression of SLP-2 in gastric adenocarcinoma tissues with lymph nodes metastasis was higher than that in gastric adenocarcinoma tissues without lymph nodes metastasis （X2 =3. 994, P〈0.05）. There was no correlation between the expression and gender, age, tumor size and differentiation degree （all P〉0.05）. After SLP 2 siRNA transfection, the expression of SLP-2 in SGC7901 cells significantly decreased at both mRNA and protein level. At 48 h after SLP 2 siRNA transfeetion, the cell migration ability was significantly lower （migrated cell number 53.0±10.8） than that of negative control group （75.8±13.4） and blank control group （78. 4±15. 2） （t 22.778 and 25.444, both P〈0.01）. The in vitro invasion ability of SGC7901 cells （migrated cell number 36. 3±8. 9）

关 键 词：胃肿瘤 腺癌 基因表达 RNA 小分子干扰 肿瘤转移 信使 肿瘤细胞 

分 类 号：R735.2[医药卫生—肿瘤]