人类真核翻译延长因子1A2过表达对胰腺癌SW1990细胞体外侵袭及体内转移的影响  

Effects of homo sapiens eukaryotic translation elongation factor 1 alpha 2 over expression on in vitroinvasion and in vivo metastasis of pancreatic cancer SW1990 cells

作　　者：许朝[1] 胡端敏[1] 章永平[1] 诸琦[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化内科,上海200025

出　　处：《中华胰腺病杂志》2013年第1期16-19,共4页Chinese Journal of Pancreatology

摘　　要：目的探讨人类真核翻译延长因子1A2（EEFlA2）过表达对人胰腺癌SW1990细胞体外侵袭和肺转移潜能的影响。方法通过携带EEFlA2的慢病毒感染人胰腺癌SW1990细胞建立EEFlA2稳定过表达的SW1990／EEFlA2细胞株及空质粒对照的SW1990／GFP细胞株，并以亲本SW1990细胞作为空白对照。采用实时PCR和蛋白质印迹法检测各组细胞EEFlA2mRAN和蛋白的表达。应用Transwell小室检测细胞的体外侵袭能力。通过裸鼠尾静脉注射癌细胞方法构建肺转移模型，8周后观察并计数肺表面的转移结节。结果SW1990／EEFlA2细胞EEFlA2mRNA相对表达量为3．252±0．344，显著高于SW1990／GFP细胞的1．000±0．060及亲本细胞的0．944±0．041（t值分别为2．255、2．305，P值均〈0．01）；EEFlA2蛋白表达量为0．833±0．050，显著高于SW1990／GFP细胞的0．247±0．035及亲本细胞的0．273±0．041（t值分别为0．572、0．559，P值均〈0．01）；穿膜细胞数为（60±4）个，显著多于SW1990／GFP细胞的（33±4）个和亲本细胞的（26±3）个（t值分别为31．33、34．78，P值均〈0．01）；裸鼠肺转移结节显著多于SW1990／GFP细胞及亲本细胞组（5／5比1／5、1／5，P值均〈0．05）。结论EEFlA2过表达可以明显增强胰腺癌SW1990细胞的体外侵袭和肺转移能力。Objective To investigate the effects of over-expression of homo sapiens eukaryotic translation elongation factor 1 alpha 2 （EEF1A2） on in vitro invasion and lung metastasis of human pancreatic cancer SW1990 cells. Methods Letivirus-mediated delivery of EEF1A2 was used to enhance the expression of EEF1A2 gene in human pancreatic cancer SW1990 cells, the SW1990 ceils stably over-expressing EEF1A2 protein （SW1990/EEF1A2 ceils） were obtained, and the parent SW1990 cells and SW1990/GFP cells were used as the control, and the expressions of EEF1 A2 mRNA and protein were determined by Real-time PCR and Western blotting. The invasion ability of cells was determined by Transwell assay. The lung metastasis model was established by injection of SW1990 cells into the tail vein. Whole lung tissues were harvested, and visible nodules on lung surface were counted macroscopically 8 weeks later. Results The EEF1A2 mRNA expression of SW1990/EEF1A2 was 3.252± 0. 344, which was significantly higher than those in SW1990/GFP cells （ 1. 000±0. 060） and SW1990 cells （0. 944 ± 0. 041, t = 2. 255, 2. 305, P 〈 0.01 ） ; the EEF1 A2 protein expression was 0. 833 ± 0. 050, which was significantly higher than those in SW1990/GFP cells （ 0. 247 ± 0.035） and SW1990 cells （0.273± 0.041, t=0.572, 0.559, P〈0.01）. The ability of invasion of SW1990/EEF1A2 ceils was （60 ±4） cells, which was significantly higher than （33 ± 4） cells in SW1990/GFPgroup and （26 ±3） cells in SW1990 group （t =31.33, 34.78, P〈0.01）. Furthermore, SW1990/EEF1A2 cells had a much higher incidence of lung metastasis in nude miee than SW1990/GFP cells and SW1990 cells in vivo （100% vs. 20% , 20% , P 〈 0.05 ）. Conclusions EEF1A2 over-expression ean obviously increase the in vitro invasion and lung metastasis of pancreatic cancer SW1990 cells.

关 键 词：胰腺肿瘤 人类真核翻译延长因子1A2 慢病毒感染 肿瘤侵袭 肿瘤转移 

分 类 号：R735.9[医药卫生—肿瘤]