骨髓间充质干细胞移植对慢性脑缺血大鼠海马区Cdc42表达及认知功能的影响  

Transplantation of bone marrow mesenchymal stem cells increasing Cdc42 protein expression in the hippocampus and improving cognitive function of rats with chronic cerebral ischemia

作　　者：于晓云[1] 张博爱[1] 李俊敏[1] 刘宇[1] 李晓晓[1] 崔璨[1] 崔红卫[1] 

机构地区：[1]郑州大学第一附属医院神经内科,河南郑州450052

出　　处：《中国老年学杂志》2013年第4期829-831,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81000530)

摘　　要：目的观察骨髓间充质干细胞移植后慢性脑缺血大鼠海马区表达Cdc42表达的变化,探讨其对慢性脑缺血大鼠认知功能的影响。方法 SD大鼠72只,随机分为假手术组、永久性双侧颈总动脉结扎(2VO)模型组、实验组(2VO模型+干细胞移植),每组分8、10、12 w 3个时间点,每个时间点8只,用Morris水迷宫筛选出造模成功的大鼠并检测大鼠的空间记忆能力,用Western印迹和免疫组化检测大鼠海马区Cdc42的表达。结果实验组和模型组大鼠海马区Cdc42的表达随时间延长逐渐降低(P<0.05),其逃避潜伏期逐渐延长(P<0.05);较之2VO模型组,同一时间点实验组Cdc42表达明显增高(P<0.05),逃避潜伏期显著缩短(P<0.05)。结论骨髓间充质干细胞移植可显著改善大鼠认知功能,这可能与其能促进慢性脑缺血大鼠海马区Cdc42的表达有关。Objective To explore the effects of bone marrow mesenchymal stem cells transplantation on cognitive function and expression of cell division cycle 42 GTP-binding protein in hippocampus of chronic cerebral hypoperfusion rats.Methods 72 SD rats were randomly divided into sham,2VO model(permanent ligation of bilateral common carotid artery) and experimental groups(2VO model + bone marrow mesenchymal stem cells transplantation).Rats in every group were subdivided into 8,10 and 12 weeks subsets.Morris water maze was used to select rats of modeled successfully and space memory ability.Expression of Cdc42 protein in hippocampus was measured by Western blotting and immunohistochemistry.Results Expression of Cdc42 in hippocampus was reduced,escape latency was extended with time extending in experimental and model groups(P < 0.05).Compared with model group,the expression of Cdc42 in the hippocampus tissue was increased and escape latency was shortened(P < 0.05) in experimental group.Conclusions Transplantation of bone marrow mesenchymal stem cells can improve cognitive function of chronic cerebral hypoperfusion rats,which maybe due to increased expression of Cdc42 protein.

关 键 词：慢性脑缺血 CDC42 骨髓间充质干细胞 海马 

分 类 号：R743[医药卫生—神经病学与精神病学]