MR扩散加权成像在大鼠肝纤维化诊断中的价值  

Value of MR diffusion weighted imaging in the diagnosis of hepatic fibrosis of rat

作　　者：范国华[1] 龚建平[1] 沈钧康[1] 张彩元[1] 徐孝秋[1] 徐亮[1] 俞泽阳[1] 张维[1] 吴浩荣[2] 

机构地区：[1]苏州大学附属第二医院影像科,215004 [2]苏州大学附属第二医院普外科,215004

出　　处：《中华放射学杂志》2013年第2期172-177,共6页Chinese Journal of Radiology

基　　金：江苏省苏州市应用基础研究计划项目（SYS201041）;苏州大学附属第二医院博士、留学归国人员预研项目（SDFEYBS1107）

摘　　要：目的探讨MRDWI对大鼠肝纤维化早期诊断及其分期的价值。方法采用四氯化碳法建立大鼠肝纤维化模型，大鼠给药后第2周开始，采用完全随机设计随机数字表抽样方法每周抽取模型组大鼠5～6只、对照组大鼠1～2只进行DWI检查，至第16周结束，共对72只肝纤维化成模组及30只对照组大鼠进行了DWI检查，测算不同b值（分别为0、300、600、800和1000s／mm^-2）时DWI的信号强度、ADC值和指数表观扩散系数（EADC）值。行DWI后4h内处死大鼠，行病理检查，成模大鼠按肝纤维化病理分期结果进行分组。不同b值的肝脏信噪比（SNR）比较，以及对照组与模型组、模型组不同分期组间ADC值和EADC值的比较采用完全随机设计资料秩转换的方差分析及SNK法多重比较，SNR与b值的相关性，以及肝纤维化分期与ADC值、EADC值的相关性采用秩相关分析。结果（1）模型组大鼠随着肝纤维化分期的增加，DWI信号强度呈增加趋势。（2）随着b值的增大，SNR呈下降趋势。b值为600、800s／mm^-2时DWI效果较理想，SNR分别为31．2±11．6和24．8±6．8。（3）72只肝纤维化组大鼠中，肝纤维化病理分期1期17只、2期21只、3期19只、4期15只。对照组、肝纤维化1～4期大鼠的ADC值分别为（1．65±0．38）X10^-3、（1．34±0．32）X10、（1．04±0．20）×10^-3、（0．994±0．19）×10^-3和（0．62±0．21）×10^-3mm^-2／s，EADC值分另0为（0．31±0．08）×10^-3、（0．34±0．09）×10^-3、（0．41±0．08）×10^-3、（0．46±0．08）×10^-3和（0．59±0．11）×10^-3mm^-2／s，差异均有统计学意义（F值分别为40．97和25．04，P值均〈0．01）。各组ADC值两两比较，除肝纤维化2期与3期比较差异无统计学意义外（P〉0．05），其余各组差异均有统计学意义（P〈0．05）。EADC值两两比较，除对照组与肝纤维化1期比较，肝纤维化2期与3期比较，差异无统计学�Objective To investigate the value of DWI in the early diagnosis and staging of hepatic fibrosis of rats. Methods Rat model with hepatic fibrosis was established by using carbon tetrachloride. By using completely randomized design random digits table ,5 or 6 rats were assigned to model group and 1 or 2 rats to the control group. After drug administration from the second week to the end of 16th weeks,a total of 72 rats of model group and 30 rats of control group were included in this study to undergo DWI scanning. The signal intensity of DWI, the value of ADC and exponential apparent diffusion coefficient （EADC） for different b values （ b = 0, 300, 600, 800 and 1000 s/mm^2 respectively ） were calculated. The rats were sacrificed to death to have pathologic examination in 4h after DWI scanning. Seventy-two model rats with hepatic fibrosis were grouped according to their pathological staging. The difference of the value of hepatic signal-to-noise ratio （SNR） of different b values,and the value of ADC and EADC among control group and model groups were compared by analysis of variance with completely randomized design data rank transformation and SNK multiple comparison. The correlation of SNR and b values, and staging of hepatic fibrosis and the value of ADC and EADC were tested by rank correlation analysis. Results （ 1 ） The signal intensity of DWI of the rats of model group showed the tendency of increase with the increase of stage of hepatic fibrosis. （2） SNR showed the tendency of decrease with the increase of b values. When b value was 600 s/mm^2 or 800 s/mm^2 , SNR was 31.2 ± 11.6 and 24. 8 ± 6.8 respectively and the quality of images was good. （3）There were 17 rats in grade 1,21 rats in grade 2,19 rats in grade 3 and 15 rats in grade 4 of hepatic fibrosis. The value of ADC of control group and of grade 1 to 4 of hepatic fibrosis were （ 1.65 ± 0.38） ×10^-3,（1.34±0.32） ×10^-3,（1.04±0.20） ×10^-3,（0.99±0.19） ×10^-3,（0.62±0.21） × 10-3 mm^2/s respectively,and t

关 键 词：大鼠 肝脏 纤维化 磁共振成像 

分 类 号：R575.2[医药卫生—消化系统]