短小芽孢杆菌产碱性纤维素酶的发酵工艺优化  

Optimization of Fermentation Process of Bacillus pumilus Producing Alkalescence Cellulase

作　　者：黄谚谚[1] 吴华珠[1] 许旭萍[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《贵州农业科学》2012年第5期172-175,共4页Guizhou Agricultural Sciences

基　　金：福建省教育厅项目(JB09029;JB11011)

摘　　要：为了确定短小芽孢杆菌(Bacillus pumilus S12)产碱性纤维素酶的最适发酵条件,采用液体摇瓶的发酵方法,进行最适发酵条件的单因素试验和正交试验。结果表明,Bacillus pumilus S12菌株合成纤维素酶的最适碳源为CMC和麸皮,最适氮源为酵母膏,有机氮比无机氮更有利于产酶;最适产酶条件为起始pH 8.5,培养温度32℃,装液量为250mL三角瓶装70mL培养液,接种量为3%,在此条件下发酵20h,酶活力可达367.9μg/mL。With the purpose of getting the optimum fermentation condition of alkalescence cellulase produced by B.pumilus S12,the fermentation condition was optimized through the single factor and orthogonal design.The results showed that the bran and CMC-Na were the suitable carbon source and the best nitrogen source was yeast.The enzyme producing rate of organic nitrogen sources was higher than that of inorganic nitrogen sources.The optimal conditions were as follows： initial pH 8.5,culture temperature 32℃,70 mL media filled in the 250 mL triangle flask,the inoculation amount 3%（v/v）,fermentation time 20 h.The alkaline cellulase activity reached 367.9 g/mL under the optimal condition.

关 键 词：短小芽孢杆菌 碱性纤维素酶 发酵工艺 

分 类 号：S182[农业科学—农业基础科学]