转染CTGF基因对乳腺癌细胞MMP-2及TIMP-2表达的影响  

Effect of gene CTGF transfection on the expression of MMP-2 and TIMP-2 in human breast cancer cells

作　　者：许斌[1] 陈寅[2] 戴桂红[2] 蒋小芹[2] 王薇[2] 赵国军[2] 于鸿[2] 

机构地区：[1]泰州市人民医院普外科,江苏泰州225000 [2]泰州市人民医院病理科,江苏泰州225000

出　　处：《江苏大学学报（医学版）》2012年第2期97-100,共4页Journal of Jiangsu University：Medicine Edition

基　　金：南通大学自然科学基金资助项目(10Z088);泰州市"311工程"人才基金项目(201036)

摘　　要：目的:通过研究结缔组织生长因子(CTGF)基因对人乳腺癌MCF-7细胞株基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶组织抑制因子-2(TIMP-2)表达的影响,深入探讨CTGF在乳腺癌侵袭和转移中的作用机制。方法:采用脂质体介导法,将CTGF基因瞬时转染体外培养的人MCF-7细胞,并用免疫荧光、RT-PCR和蛋白质印迹法检测其转染成功与否;再采用蛋白质印迹法检测转基因MCF-7 MMP-2及TIMP-2表达改变。结果:与对照组相比,转染CTGF基因的MCF-7,其CTGF mRNA和蛋白表达均显著增高,MMP-2蛋白表达水平显著增高,而TIMP-2蛋白表达水平显著降低。结论:CTGF可能通过增加乳腺癌细胞MMP-2表达及抑制TIMP-2的生成而起到促进乳腺癌侵袭和转移的作用。Objective： To investigate the effect of connective tissue growth factor （CTGF） on the expres- sion of matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metalloproteinase-2 （TIMP-2） in human breast cancer line MCF-7. This study was designed to explore the underlying mechanism for the role of CT- GF in the development of breast cancer. Methods： Lipofectin method was used to transfect CTGF vectors into MCF-7; immunofluorescence, RT-PCR and Western blot analysis were used to detect their transfeetion efficiency. The expressions of MMP-2 and TIMP-2 were determined by Western blot analysis. Results： Im- munofluorescence, RT-PCR and Western blot analysis showed a successful transfection of CTGF vectors into MCF-7. MCF-7 transfected with CTGF gene showed an increased expression of MMP-2 protein significantly, compared with control groups. On the other hand, MCF-7 transfected with CTGF gene showed a decreased expression of TIMP-2 protein significantly, compared with control groups. Conclusion： It is possible that CTGF could promote the development of breast cancer by effectively enhancing the expressions of MMP-2 and TIMP-2 in MCF-7 cells.

关 键 词：结缔组织生长因子 基质金属蛋白酶-2 基质金属蛋白酶组织抑制因子-2 

分 类 号：R73-362[医药卫生—肿瘤]