海洋真菌PenicilliumoxalicumSCSGAF0023的次生代谢产物及其抗污损和酶抑制活性研究  

Secondary Metabolites of Marine-derived Fungus Penicillium oxalicum SCSGAF 0023 and Their Antifouling and Enzyme-inhibitory Activities

作　　者：孙玉林[1,2] 陈茵 徐新亚 张晓勇[1] 郑智慧[4] 农旭华[1,2] 鲍洁[1,2] 漆淑华[1] 

机构地区：[1]中国科学院南海海洋研究所中国科学院海洋生物资源可持续利用重点实验室,广州510301 [2]中国科学院大学,北京100049 [3]深圳市第九人民医院,深圳518116 [4]华北制药集团新药研究开发有限责任公司,石家庄050011

出　　处：《天然产物研究与开发》2013年第1期1-6,59,共7页Natural Product Research and Development

基　　金：国家973前沿领域项目(2010CB833800);国家自然科学基金项目(40931160435,40976090)

摘　　要：利用pTLC、硅胶、Sephadex LH-20及半制备HPLC等柱色谱手段,从海洋真菌Penicillium oxalicum SCS-GAF 0023的次生代谢产物中分离得到16个化合物,波谱学数据分析鉴定为n-butyl isobutyl terephthalate(1),dibutyl terephthalate(2),1,3,7-trihydroxy-6-methylanthraquinone(3),1,6,7-trihydroxy-3-methoxy-anthraquinone(4),isorhodoptilometrin(5),citreorosein(6),emodin(7),methyl-3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate(8),pinselin(9),secalonic acid D(10),quinolactacin C1(11),quinolactacin C2(12),quinolactacin A1(13),quinolactacin A2(14),quinolactacin B1(15),3-methyl-1H-indole-2-carboxylic acid(16)。其中,化合物1为新天然产物,化合物5和7表现出强的抗草苔虫幼虫附着活性,EC50值分别为3.8和6.0μg/mL;化合物10和15分别对PTPIB和cathepsin B显示中等的酶抑制活性,IC50值分别为24.0和16.0μM。Sixteen compounds were isolated from marine-derived fungus Penicillium oxalicum SCSGAF 0023 strain. They were identified to be n-butyl isobutyl terephthalate （ 1 ）, dibutyl terephthalate （2）, 1,3,7-trihydroxy-6-methylanthra- quinone （3）, 1,6,7-trihydroxy-3-methoxy-anthraquinone （ 4 ）, isorhodoptilometrin （ 5 ）, citreorosein （ 6 ）, emodin （7）, methyl-3,8-dihydroxy-6-methyl-9- oxo-9H-xanthene-1 -carboxylate （ 8 ）, pinselin （ 9 ）, secalonic acid D （ 10 ）, quinolactaein C1 （ 11 ）, quinolactacin C2 （ 12）, quinolaetacin A, （ 13）, quinolactacin A2 （ 14）, quinolactaein B, （ 15 ）, and 3-methyl-1H-indole-2-carboxylie acid （16） on the basis of spectroscopic analysis and comparison with literatures. Compound I was a new natural product. Compounds 5 and 7 showed strong antifouling activity with ECso values of 3.8 and 6.0 μg/ml, respectively. Compound 10 exhibited moderate inhibitory activity against PTPIB with IC50 value of 24.0 μM, and 15 showed moderate inhibitory activity against cathepsin B with IC50 value of 16.0 μM.

关 键 词：海洋真菌 PENICILLIUM oxalicum SCSGAF 0023 次生代谢产物 抗污损 酶抑制活性 

分 类 号：R284.2[医药卫生—中药学]