新疆维吾尔族宫颈癌蛋白质组图谱分析  

Analysis on proteome among cervical cancer patients of Uighur nationality in Xinjiang

作　　者：陈锐[1] 石瑛[2] 彭靖淇[1] 张琼[2] 周琦[1] 

机构地区：[1]新疆医科大学附属肿瘤医院妇外四科,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院检验科

出　　处：《中国妇幼保健》2013年第6期1013-1015,共3页Maternal and Child Health Care of China

基　　金：新疆卫生厅青年专项基金〔2008y05011〕

摘　　要：目的:探讨筛选并建立新疆维吾尔族子宫颈癌血清蛋白质组图谱诊断模型可能的临床价值。方法:采用CM10蛋白芯片和SELDI-TOF-MS技术检测40例子宫颈鳞癌、30例宫颈上皮内瘤变Ⅲ级和15例健康人的血清蛋白质组图谱,ZUCI-蛋白芯片数据分析系统分析并建立维吾尔族子宫颈癌蛋白质组图谱诊断模型。结果:宫颈癌与宫颈上皮内瘤变Ⅲ级患者和健康人存在139种差异蛋白质质谱峰,有分类意义的蛋白质7种。其中质荷比为6 467.26、6 993.199在正常对照组、上皮内瘤变组和宫颈癌组中表达逐渐降低;1 081.275、1 083.193、1 122.849、8 929.189、15 939.86在正常对照组、上皮内瘤变组和宫颈癌组中表达逐渐增高。这7个质荷比峰建立了维吾尔族宫颈癌蛋白质组图谱诊断模型,其准确度85.00%,敏感度72.73%。结论:该组建立的诊断模型可以有效区分维吾尔族宫颈癌患者和正常妇女,为新疆维吾尔族宫颈癌癌的诊断与筛查提供了一条崭新途径。Objective： To explore the clinical value of screening and constructing diagnostic model of serum proteome among cervi- cal cancer patients of Uighnr nationality. Methods ： CM10 protein chip and SELDI - TOF - MS technology were used to detect serum pro- teins in 40 patients with cervical squamous cancer, 30 patients with cervical intraepithelial neoplasia （CIN）Ⅲ, and 15 healthy women, the diagnostic model of proteome among cervical cancer patients of Uighur nationality was analyzed and constructed by ZUCI - protein chip data analytic system. Results： A total of 139 kinds of differentially expressed proteins were screened out in patients with cervical cancer and CIN Ⅲ and healthy women, and 7 kinds of proteins had significance. The mass - to - charge ratios were 6 467.26 and 6 993. 199, respectively, which decreased gradually in normal control group, CIN group, and cervical cancer group; 1 081. 275, 1 083. 193, 1 122. 849, 8 929. 189, and 15 939.86, respectively, which increased gradually in normal control group, CIN group, and cervical cancer group. The diagnostic mod- el of proteome tree was constructed among cervical cancer patients of Uighur nationality based on mass - to - charge ratios of the seven kinds of proteins, the accuracy and sensitivity were 85.00% and 72. 73%, respectively. Conclusion： The diagnostic model constructed in the study can effectively differentiate cervical cancer patients of Uighur nationality from normal women, which provides a new way for diagnosing and screening cervical cancer among women of Uighur nationality.

关 键 词：宫颈癌 表面增强激光解析离子化飞行时间质谱 维吾尔族 

分 类 号：R737.33[医药卫生—肿瘤]