ALDH2和ADH1B基因多态性与食管癌淋巴结转移的相关性  

Relationship between ALDH2,ADH1B variant alleles and risk of esophageal cancer lymphatic metastasis

作　　者：方娜[1] 钟锡明[1] 范钰[1] 

机构地区：[1]江苏大学附属人民医院肿瘤科,江苏镇江212002

出　　处：《江苏大学学报（医学版）》2012年第5期414-417,共4页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:研究中国汉族人群中乙醛脱氢酶2(aldehyde dehydrogenase 2,ALDH2)和乙醇脱氢酶1B(alcohol dehy-drogenase 1B,ADH1B)基因多态性与食管癌淋巴结转移风险性的关系。方法:采用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization-time of flight mass spectrometry,MALDI-TOF MS)技术分析85例有淋巴结转移的食管癌和270例无淋巴结转移的食管癌标本ALDH2 rs671和ADH1B rs1229984基因多态性,计算各种基因型与食管癌淋巴结转移的相对风险度及其95%可信区间。结果:ALDH2 rs671 G>A3种多态基因型GG,GA,AA在食管癌淋巴结转移组和无淋巴结转移组的频率分别为58.8%(GG),38.8%(GA),2.4%(AA)和59.3%(GG),37.4%(GA),3.3%(AA)(χ2=0.237,P=0.888);以ALDH2 rs671 GG基因型作参照,ALDH2 rs671 AA基因型食管癌淋巴结转移发生的风险降低(调整年龄、性别、吸烟及饮酒状态OR=0.70,95%CI=0.14～3.45),但相关性未达到明显统计学意义。ADH1B rs1229984 A>G3种多态基因型AA,AG,GG在食管癌淋巴结转移组和无淋巴结转移组的频率分别为38.8%(AA),50.6%(AG),10.6%(GG)和42.8%(AA),41.6%(AG),15.6%(GG)(χ2=2.553,P=0.279),以ADH1B rs1229984 AA基因型作参照,ADH1B rs1229984 GG基因型食管癌淋巴结转移发生的风险降低(调整年龄、性别、吸烟及饮酒状态OR=0.66,95%CI=0.29～1.53),但相关性未达到明显统计学意义。结论:ALDH2 rs671G>A和ADH1B rs1229984 A>G基因多态性可能与食管癌淋巴结转移的风险无明显相关,需进一步研究证实。Objective： To investigate the relationship between ADH1B rsl229984 and ALDH2 rs671 polymorphisms and the susceptibility to lymphatic metastasis in esophageal cancer in a Chinese Han popula- tion. Methods： Genotypes were determined by matrix-assisted laser desorption/ionization-time of flight mass spectrometry（ MALDI-TOF MS） method in 85 esophageal cancer lymphatic metastasis positive patients and 270 lymphatic metastasis negative patients. The associations between ALDH2 rs671 G 〉 A and ADH1B rs1229984 A 〉 G genotypes and the risk of esophageal cancer lymphatic metastasis were estimated by com- puting the odds ratio（OR） and their 95% confidence interval（CI）. Results： The ALDH2 rs671 G 〉 A genotype frequencies were 58.8% （GG） , 38.8% （ GA）, 2.4% （AA） in the lymphatic positive group and 59.3% （ GG）, 37.4% （ GA）, 3.3% （AA） in the lymphatic negative group, respectively（X^2 = 0.237, P = 0. 888 ） ; logistic regression analyses revealed that ALDH2 rs671 AA genotype could reduce the risk of lym- phatic metastasis（adjusted OR =0.70, 95% CI = 0.14 - 3.45） compared with ALDH2 rs671 GG geno- type, but the difference was not statistically significant. The ADH1B rs1229984 A 〉 G genotype frequencies were 38.8% （AA）, 50.6% （AG）, 10.6% （GG） in the lymphatic positive group and 42.8% （AA）, 41.6% （AG）, 15.6% （GG） in the lymphatic negative group, respectively （X^2 = 2. 553, P = 0. 279） ; logistic regression analyses revealed that ADH1B rs1229984 GG genotype could reduce the risk of lymphaticmetastasis（ adjusted OR = 0.66, 95% CI = 0.29 - 1.53 ） compared with ADH1B rs1229984 AA genotype, but the difference was not statistically significant. Conclusion： ALDH2 rs671 G 〉 A and ADH1B rs1229984 A 〉 G polymorphisms might not be associated with the risk of esophageal cancer lymph node me- tastasis, and awaits further investigation.

关 键 词：乙醛脱氢酶2 乙醇脱氢酶1B 食管癌 淋巴结转移 单核苷酸多态性 

分 类 号：R735.1[医药卫生—肿瘤]