RNA干扰PP2A-Aα基因表达对结直肠癌细胞生物学行为的影响  

Biological behaviors of colorectal cancer cells influenced by silencing PP2A-Aα using RNA interference technique in vitro

作　　者：白雪[1] 李世拥[1] 于波[1] 杜峻峰[1] 魏晓军[1] 蔡慧云[1] 

机构地区：[1]北京军区总医院普通外科全军普通外科中心,100700

出　　处：《中华普外科手术学杂志（电子版）》2012年第2期31-34,共4页Chinese Journal of Operative Procedures of General Surgery(Electronic Edition)

基　　金：国家自然科学基金(No.81101860 and No.81000189)

摘　　要：目的探讨RNA干扰PP2A-Aα基因表达对结直肠癌细胞生物学行为的影响。方法设计了3条shRNA干扰载体并分别稳定的转染结直肠癌SW480细胞。RT-PCR方法筛选出一组干扰效率最高的转染细胞进行进一步实验。应用MTT法检测干扰后结直肠癌细胞的增殖能力,应用Transwell实验检测干扰后结直肠癌细胞的运动侵袭能力。结果转染pS-shRNA599干扰载体的SW480细胞PP2A-Aα基因表达明显降低,抑制率达72.8%。应用MTT法检测干扰后结直肠癌细胞的增殖能力明显增强(P<0.01);Transwell实验显示,干扰组细胞的穿膜细胞数明显多于对照组细胞(P<0.01)。结论 PP2A-Aα基因表达降低可明显提高结直肠癌SW480细胞的增殖能力与运动侵袭能力,PP2A在结直肠癌细胞中可能扮演着肿瘤抑制剂的角色。Objective To investigate the influence of the PP2A-Aα gene on the biological behaviors of colorectal cancer cells. Methods Three shRNA vectors were designed and stably transfected into SW480 cells. RT-PCR analysis was made to screen the group with the highest inhibitory rate. The cellular proliferation capacity was detected by MTT assay and the motion invasion capacity was detected by transwell assay of colorectal cancer SW480 cells after stable transfection. Results RT-PCR analysis showed that cancer cells transfected with pS-shRNA599 vector had the strongest inhibition of PP2A-Aα expression （72.8%）, while MTY assay showed that the cellular proliferation capacity of cancer cells in the experimental group was enhanced more significantly than that in the control group （ P 〈 0.01 ） . The transwell assay showed that the cells passing the polycarbonate membranes were more significant in PP2A-Aα-knockdown cells than in the control cells （P 〈 0.01 ）. Conclusions Reduction of PP2A-Aα mRNA expression could improve the capacity of cellular proliferation and invasion of eolorectal cancer SW480 cells. PP2A might play a tumor suppressive role in colorectal cancer ceils.

关 键 词：结直肠肿瘤 蛋白质磷酸酶2 RNA干扰 基因表达 

分 类 号：R735.3[医药卫生—肿瘤]