分子对接和动力学模拟提高嗜热蛋白酶PhpI的活力  

Molecular Docking and Dynamics Simulation Improving Thermophilic Protease Activity of PhpI

作　　者：詹冬玲[1,2] 高楠[3] 韩葳葳[1] 冯雁[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130012 [2]吉林农业大学食品科学与工程学院,长春130118 [3]中国科学院长春应用化学研究所,长春130022

出　　处：《高等学校化学学报》2013年第3期628-633,共6页Chemical Journal of Chinese Universities

基　　金：国家'九七三'计划项目(批准号:2012CB721003);国家自然科学基金(批准号:31070638)资助

摘　　要：通过分子对接和动力学模拟对嗜热蛋白酶的分子进行改造,确定蛋白酶PH1704(PhpI)定点突变残基,并通过分子生物学实验进行验证.突变体K43C的蛋白酶活力提高了5.8倍.分子动力学模拟结果表明,经过8 ns的动力学模拟后,K43C突变体二级结构由野生型的S2片层(F11-E12-D13)变成环状结构.E12和K43均是活性位点的重要残基,这种变化将导致活性位点的柔性增强,有利于催化反应的发生.In this study,flexible docking approach was employed to dock the substrate into the active site of protease PH1704（PhpI）,combining with crystal structure to determine the key enzyme,and study on site-mutation in theroy.All parameters were verified by molecular biology experiment.The protease activity of K43C was 5.8 times higher than that of WT.Molecular dynamics simulation reveals that after 8 ns dynamics simulations,the secondary structure of K43C mutant changes from the S2 sheet of WT（F11-E12-D13）to the loop.K43 and E12 are both the important active site residues.The change will enhance the flexibility of active site,and be helpful for catalytic reactions.The new structural and mechanistic insights obtained from molecular docking and dynamics simulation should be valuable for detailed researching of structures and mechanisms of the member of the DJ-1 superfamily.

关 键 词：嗜热蛋白酶PhpI 分子对接 动力学模拟 定点突变 

分 类 号：O641[理学—物理化学] Q556[理学—化学]