傅立叶变换红外光谱技术对5种沙门氏菌的快速分类鉴定  

Rapid differentiation and identification of five species of Salmonella by FT-IR spectroscopy

作　　者：杨丽君 李兆杰 宋晓华 王静 刘玉敏 崔凤杰 

机构地区：[1]威海出入境检验检疫局,山东威海264205 [2]烟台出入境检验检疫局,山东烟台264200

出　　处：《微生物学通报》2013年第2期373-379,共7页Microbiology China

基　　金：国家质量监督检验检疫总局科研项目(No.2010IK151)

摘　　要：【目的】建立沙门氏菌属内鼠伤寒沙门氏菌、肠炎沙门氏菌、猪霍乱沙门氏菌、亚利桑那沙门氏菌、波斯坦沙门氏菌5种菌的傅立叶变换红外(Fourier transform infrared,FT-IR)光谱数据库及FT-IR分类鉴定方法。【方法】应用FT-IR技术对5种沙门氏菌进行指纹图谱数据采集,应用化学计量学分析方法对光谱进行分析。【结果】建立了5种沙门氏菌的标准FT-IR光谱数据库,用于FT-IR技术对5种可疑目标沙门氏菌进行鉴定;建立了基于主成分分析(Principal component analysis,PCA)和分级聚类分析(Hierarchical cluster analysis,HCA)两种聚类分析模型,均可成功将5种沙门氏菌进行区分。【结论】傅立叶变换红外光谱分析方法简便、快速、易操作,结果重现性好,是一种区分5种沙门氏菌的有效方法。[Objective]To establish a standard fourier transform infrared(FT-IR) spectral li-brary and a FT-IR method of differentiation and identification of five species of Salmonella,viz Salmonella typhimurium,Salmonella enteritidis,Salmonella choleraesuis,Salmonella arizonae and Salmonella potsdam.[Methods]FT-IR fingerprint absorption spectra of five species of Salmonella were collected and analyzed by chemometric methods.[Results]A standard FT-IR spectral library was created and could be used to compare with those of the target Salmonella and identify them;two cluster models of principal component analysis(PCA) and hierarchical cluster analysis(HCA) were established and could differentiate and identify well the five species of Salmonella.[Conclusion]As a rapid,easy-to-use and accurate technique,FT-IR spectroscopy is an effective tool to differentiate and identify five species of Salmonella studied here.

关 键 词：傅立叶变换红外光谱 沙门氏菌 主成分分析 分级聚类分析 分类 鉴定 

分 类 号：Q939[生物学—微生物学]