猪苓硒多糖连续合成工艺及抑菌性能研究  

Study on the continuous chemical synthesis process of polyporus selenium-polysaccharide and its bacteriostasis

作　　者：李志洲[1] 刘军海[1] 王俊宏[1] 邓百万[2] 李丽华[1] 

机构地区：[1]陕西理工学院化学与环境科学学院,陕西汉中723000 [2]陕西理工学院生物科学与工程学院,陕西汉中723000

出　　处：《食品与机械》2013年第1期31-35,共5页Food and Machinery

基　　金：陕西省教育厅科研资助项目(编号:11JK0596)

摘　　要：采用化学合成法,以猪苓多糖和亚硒酸钠为原料制备猪苓硒多糖。试验设计连续式合成工艺流程,并对硒多糖的连续式超声波辅助化学合成工艺条件进行研究;对所制备的硒多糖进行抑菌性能研究,并与猪苓多糖进行比较。结果表明,猪苓多糖、氯化钡、亚硒酸钠溶液浓度均为0.2mg/mL,采用连续式合成猪苓硒多糖的最佳工艺条件为多糖进料流量30 mL/min,亚硒酸钠溶液与猪苓多糖溶液流量配比1∶1,反应温度70℃、反应釜内硝酸的浓度0.008mL/mL,超声辅助合成频率为30kHz,其合成率达26.5mg/g;抑菌性能研究表明,猪苓硒多糖对大肠杆菌、金黄色葡萄球菌、啤酒酵母和黑曲霉均有抑制作用,且其抑菌能力明显优于猪苓多糖。The polyporus selenide-polysaccharides was synthesized by the chemical synthesis principle using the polyporus polysaccharides and sodium selenite for the raw materials. The process of continuous chemical synthesis was designed to selenium polysaccharide, and the technological conditions of the continuous chemical synthesis process were researched under ultrasonic assisting impact. The antimicrobial activity of preparative selenide polysaccharides was studied and com- paring to the polysaccharides from polyporus. The result showed that under the raw materials solution concentration of the polyporus polysaccharides, barium chloride for catalyst and sodium selenite were 0.2 mg/mL, the optimized preparation technique conditions were the ultrasonic assisting frequency 30 kHz, the flow of polyporus polysaccharides solution 30 mL/min, the flow proportioning of the polyporus polysaccharides and sodium selenite solution to 1 ： 1, reac- tion temperature 70 ℃, the solution concentration of nitric acid in reaction fill 0. 008 mL/mL, and the synthetic ratio was 26. 5 mg/g. The research of bacteriostasis property showed that Polyporus Sele- nium polysaccharide could produce prominent antibiotic action to Escherichia coli , Staphylococcus aureus , Saccharom yces cerevisiae and Aspergillus niger; and it ＇s antibiotic action was superior to pol- yporus polysaccharides.

关 键 词：猪苓 多糖 硒多糖 连续式 抑菌作用 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]