TNF-α和IL-1β对人脐静脉内皮细胞蛋白C受体mRNA表达的影响  

Effects of TNF-α and IL-1β on EPCR mRNA in endothelial cells

作　　者：郭小芳 刘海波 任惠龙 龚维坤 成兴波[2] 

机构地区：[1]浙江省宁波市鄞州人民医院,宁波315040 [2]苏州大学医学院附属第一医院

出　　处：《浙江中西医结合杂志》2013年第2期98-101,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:观察肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)对人脐静脉内皮细胞(ECV304)蛋白C受体(EPCR)mRNA表达的影响。方法:通过体外培养ECV304细胞,分别以含TNF-α和IL-1β的培养基孵育ECV304细胞,行剂量和时间依赖性实验。采用实时定量聚合酶链反应(quantitative real-time PCR)技术测定ECV304细胞EPCR mRNA的表达。结果:TNF-α和IL-1β均能显著下调ECV304 EPCR mRNA的表达,并呈剂量和时间依赖性(P<0.05)。结论:TNF-α和IL-1β可能通过显著下调ECV304上EPCR mRNA的表达而成为损伤内皮细胞功能的一个新作用机制。Objective： To investigate the effect of tumor necrosis factor alpha （TNF-α and interleukin-1 beta （IL-1β） on the expression of endothelial protein C receptor （EPCR） in human umbilical vein endothelial cells （ECV304）. Methods： Cultured ECV304 were used as target ceils. Different concentrations of TNF-α or IL-1β were added to the culture medium to establish the injury model of ECV304. EPCR mRNA were measured by quantitative real-time PCR. Results： The expression of EPCR in ECV304 was down-regulated by TNF-α and IL-1β in dose and time dependent manner. Conclusion： Both TNF-α and IL-1β may damage the function of endothelial cells by down-regulating the expression of EPCR mRNA.

关 键 词：内皮细胞肿瘤坏死因子 白细胞介素-1 内皮细胞蛋白C受体 

分 类 号：R363[医药卫生—病理学]