β-葡萄糖苷酶高产菌株及其应用研究  

Application Studies on High Activity β-Glucosidase Producing Strain

作　　者：梁翠谊[1] 许敬亮[1] 袁振宏[1] 张宇[1] 徐惠娟[1] 庄新姝[1] 高月淑[1,2] 

机构地区：[1]中国科学院广州能源研究所∥中国科学院可再生能源与天然气水合物重点实验室,广东广州510640 [2]中国科学院研究生院,北京100039

出　　处：《中山大学学报（自然科学版）》2013年第1期118-122,129,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家科技支撑计划资助项目(2011BAD22B01);国家自然科学基金资助项目(21176237;21211140237);国家高新技术研究发展计划(863计划)资助项目(2013AA065803)

摘　　要：对Hypocrea sp. W63的β-葡萄糖苷酶酶学性质研究表明,该酶液体发酵最高酶活高达482.1 U/mL,最适反应pH值为4.8,最适反应温度为65℃;乙醇体积分数为10%时对酶活有最大促进效果,乙醇耐受能力高达30%;将该酶应用于同步糖化发酵研究中,发现发酵至120 h乙醇质量浓度可高达41.25 g/L,与对照相比,乙醇产量均提高近2倍。该菌株所产的β-葡萄糖苷酶较高的酶活力、耐热性能、乙醇耐受性和同步糖化发酵促进效果,预示着该菌在纤维素乙醇产业化应用中具有广阔的应用前景。Studies of β -glucosidase enzymatic properties from Hypocrea sp. W63 show that, enzyme ac- tivity determined by pNPG （4 - nitrophenyl β - D - glucopyranoside） can attain to 482. 1 U/mL. The optimum reaction pH value and temperature are 4. 8 and 65 , respectively. 10% of ethanol has the greatest promotion, the β-glucosidase activity can nearly increased double and the ethanol tolerance of β -glucosidase can attain to 30%. When simultaneous saccharification and fermentation （SSF） carried out for 120 h with β - glucosidase adjunction, 41.25 g/L of ethanol can be obtained. Compared with control, the ethanol production can increase nearly 2 - fold. The β - glucosidase with high enzyme activi- ty, thermostable and alcohol tolerance for SSF application, indicate that the β - glucosidase producing strain has great potent application on cellulosic ethanol production.

关 键 词：Β-葡萄糖苷酶 耐高温 耐乙醇 同步糖化发酵 

分 类 号：Q936[生物学—微生物学] Q939.9