rhKD/APPvar对实验性慢性肝损伤肝组织纤维化影响的研究  

Influence of rhKD/APPvar on the fibrosis of hepatic tissue in Wistar rats with chronic liver injury

作　　者：孟威宏[1] 王虹蛟 王强 颜炜群[3] 任立群[3] 

机构地区：[1]沈阳军区总医院,辽宁沈阳110015 [2]长春解放军第461医院 [3]吉林大学再生医学科学研究所

出　　处：《中国实验诊断学》2013年第2期255-258,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然基金课题(课题编号30300153)

摘　　要：目的探讨重组人淀粉样蛋白前体Kunitz型蛋白酶抑制剂结构域变异体(reorganization human Kunitzprotease inhibitor domain of amyloid protein precursor variant,rhKD/APPvar)对实验性慢性肝损伤大鼠肝组织纤维化的影响。方法利用实验鼠的四氯化碳(CCl4)慢性肝损伤模型,采用胶原纤维染色检测方法,观察rhKD/APPvar对慢性肝损伤大鼠肝组织纤维化改变的影响。结果随着rhKD/APPvar剂量的增加,胶原纤维增生减轻。尤其以rhKD/APPvar大剂量组效果最为明显。结论 rhKD/APPvar能够显著抑制大鼠肝脏纤维化。Objective To investigate the influence of reorganization human Kunitz protease inhibitor domain of amy- loid protein precursor variant （rhKD/APPvar） on the fibrosis of hepatic tissue in Wistar rats with chronic liver injury. Methods The chronic liver injury model of rats was induced by carbon tetrachloride （CC14）. The method of collage- nous fibers dyeing was employed to observe the effect of rhKD/APPvar on the rats＇ hepatic tissue fibrosis. Results With the increase of the dose of rhKD/APPvar,the collagenous fibers hyperplasia decreased. The large dose of rhKD/ APPvar group was most effective in reducing fibrosis. Conclusion The rhKD/APPvar could inhibit rats＇ hepatic tissue fibrosis significantly.

关 键 词：rhKD APPvar 四氯化碳 慢性肝损伤 大鼠 胶原纤维染色 

分 类 号：R575.2[医药卫生—消化系统]