牛瑟氏泰勒虫热休克蛋白70的克隆与原核表达重组质粒的构建  

Cloning and Construction of Recombinant Prokaryotic Expression Plasmid of Theileria sergenti HSP70 Gene

作　　者：王轶男[1] 贾立军[1] 薛书江[1] 胡诗悦[1] 钱年超[1] 张守发[1] 

机构地区：[1]延边大学农学院动物医学系,吉林延吉133002

出　　处：《动物医学进展》2013年第2期60-63,共4页Progress In Veterinary Medicine

基　　金：吉林省自然科学基金项目(201115230)

摘　　要：为了构建牛瑟氏泰勒虫热休克蛋白70(HSP70)基因片段原核重组表达质粒,本试验根据Gen-Bank上登录的牛瑟氏泰勒虫热休克蛋白70基因序列(D12692.1),应用Primer Premier 5.0软件设计合成一对特异性引物,以牛瑟氏泰勒虫DNA为模板,扩增片段大小为1 692bp,与参考序列的同源性为99%。将该基因与pET-28a(+)载体进行连接,成功构建了重组表达载体pET-28a-HSP70,本试验为进一步研究牛瑟氏泰勒虫HSP70基因佐剂作用、研制基因工程疫苗奠定了基础。To construct the recombinant prokaryotic expression plasmid expressing heat shock protein 70 （HSP70） gene of Theileria sergenti, a pair of specific primers were designed and synthesized according to heat shock protein70 sequence of Theileria sergenti in GenBank （D12692.1）. T. sergenti DNA was used as template. 1 692 bp fragments was amplified. The fragment shared 98%homology with the reference sequence. The gene was subcloned into pET-28a （＋） vector to construct the recombinant expression vector pET-28a-HSP70. This research set a solid foundation for the research on the adjuvant function of HSP70 gene and the vaccine against T. sergenti.

关 键 词：牛瑟氏泰勒虫 热休克蛋白70 重组质粒 

分 类 号：S854[农业科学—临床兽医学]