Stenotrophomonas maltophilia产角蛋白酶对羊毛的作用机理探讨  

Mechanism of keratinase from Stenotrophomonas maltophiliaon wool

作　　者：李贲[1] 吴建明 朱华君 王强[1] 王平[1] 范雪荣[1] 

机构地区：[1]江南大学生态纺织教育部重点实验室,无锡214122 [2]宁波雅戈尔毛纺织染整有限公司,宁波315192 [3]无锡协新毛纺织有限公司,无锡214192

出　　处：《生物学杂志》2013年第1期47-50,70,共5页Journal of Biology

基　　金：国家自然科学基金项目(51073073);教育部长江学者和创新团队发展计划(No.IRT1135);江苏省企业研究生工作站研究项目(苏教研〔2011〕13号);江苏高校优势学科建设工程资助项目(〔苏政办发2011〕137号);江苏省科技支撑计划项目(BE2012019)

摘　　要：采用Stenotrophomonas maltophilia产角蛋白酶降解羊毛角朊蛋白,通过测定反应前后残液中巯基和肽键的变化探讨角蛋白酶作用机理。结果表明,角蛋白酶主要作用是断裂角蛋白中的二硫键,也能降解大分子蛋白质,但作用效果不强。羊毛胱氨酸分析结果进一步证明角蛋白酶能断裂羊毛鳞片层中胱氨酸二硫键。SEM结果显示单独使用角蛋白酶对羊毛鳞片去除效果不佳,但角蛋白酶和蛋白酶二浴法工艺能有效降解、剥离羊毛鳞片。A novel keratinase from Stenotrophomonas maltophilia was used to degrade wool keratin protein. Through the determination of the changes of hydrosulfide groups and peptide bonds in the treatment bath before and after the enzymatic reaction, the mechanism of keratinase on wool was studied. The results showed that the main role of keratinase is to break the disulfide bonds in the keratin struc- ture, followed by the slight degradation of macromolecular proteins. Amino acid analysis of wool fibers further proved that keratinase could break cystine disulfide bonds in wool scales. Scanning electron microscopy images showed the removal of wool scales with kera- tinase alone was not obvious, but the two-bath processing with keratinase and protease can effectively degrade and remove them.

关 键 词：角蛋白酶 二硫键 羊毛 作用机理 

分 类 号：Q556.9[生物学—生物化学] TS195.1[轻工技术与工程—纺织化学与染整工程]