辐射对人鼻咽低分化鳞癌细胞株(CNE-2)凋亡率和相关基因转录水平的影响  

The influence of radiation to apoptosis associated genes in poor differentiated nasopharyngeal carcinoma cells

作　　者：吴冉[1] 黄莉[1] 李晓霞[1] 徐丽[1] 王若峥[1] 

机构地区：[1]新疆医科大学附属肿瘤医院放疗科,乌鲁木齐830011

出　　处：《新疆医科大学学报》2013年第1期1-6,共6页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金(2009211B09);乌鲁木齐市人才工程重点培养对象专项基金(P112310001)

摘　　要：目的探讨辐射诱发的人鼻咽低分化鳞癌细胞株(CNE-2)凋亡率和Bak、Bid、Bcl-w、Bcl-xl转录水平的影响。方法体外培养CNE-2,应用流式细胞术及RT-PCR方法检测不同辐射剂量(0、2、4、6、8Gy)下CNE-2凋亡率及凋亡相关基因Bak、Bid、Bcl-w、Bcl-xl的转录水平。结果 CNE-2照射后Bak表达上调,与辐射剂量及晚期凋亡率呈正相关;Bcl-w表达下调,与辐射剂量及晚期凋亡率呈负相关;Bcl-xl表达下调,与辐射剂量及晚期凋亡率无相关性;Bid在不同剂量点转录水平差异均无统计学意义。结论 Bak、Bcl-w、Bcl-xl可能参与了辐射诱发的CNE-2细胞凋亡的调控。Objective To investigate the relationship between the mRNA expression of Bak, Bid, Bcl-w, Bcl-xl and radiation induced apoptosis in poor differentiated nasopharyngeal carcinoma （NPC） cell line CNE-2. Methods The NPC cell line CNE2 were in vitro culture, cellular apoptosis was measured by flow cytometry, reverse transcription polymerase chain reaction （RT-PCR） was used to detect mRNA expres sions of Bak, Bid, Bcl-w and Bcl-xl respectively before and after radiation （the dosage were 2, 4, 6, 8 Gy, respectively） with linear accelerator. Results In CNE 2, the mRNA e positively correlated with the dose of radiation and late apoptosis rate , xp th ression of Bak was increased and e mRNA expression of Bcl-w was decreased and negatively correlated with the dose of radiation and late apoptosis rate, the mRNA expression of Bcl-xl was decreased and unrelated with the dose of radiation and late apoptosis rate, the mRNA expression of Bid in different dose group was not statistically significant. Conclusion Bak, Bcl-w Bcl-xl may participate in the regulation of radiation induced apoptosis in CNE-2.

关 键 词：鼻咽癌 放射敏感性 BAK BID Bcl—w BCL xl 

分 类 号：R730.5[医药卫生—肿瘤]