3678例已婚女性宫颈细胞HPV感染基因谱的研究  

Study on human papillomavirus genetic profile in 3 678 cervical cell samples of married women

作　　者：张劲松 耿建祥[2] 韩春荣[2] 王旭波[2] 李海[2] 兰建云[2] 张昶[2] 张微[2] 赵雪[2] 

机构地区：[1]南京扬子医院感染科,江苏南京210048 [2]南京中医药大学第三附属医院病理科/江苏省HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2013年第4期439-441,共3页International Journal of Laboratory Medicine

基　　金：南京市卫生局中医专项支助项目(2009-92)

摘　　要：目的人乳头瘤病毒(HPV)感染是宫颈上皮内瘤变及宫颈癌的主要致病因子。该文旨在探讨南京地区已婚女性宫颈细胞中23种人乳头瘤病毒感染的基因谱分布情况及其临床意义。方法从南京地区扬子石化集团公司3678例已婚女性职工的宫颈上皮细胞标本中提取23种HPVDNA,采用基因扩增结合基因芯片技术对其宫颈细胞进行23种HPV基因型的检测,并对其受检者进行相关资料分析。结果 3678例已婚女性宫颈上皮细胞标本中检出HPV感染者397例,总的HPV感染率为10.79%(397/3678),其中单一型别的阳性检出率为9.11%(335/3678);单一型别的感染中HPV43型为80例,其阳性检出率为2.18%(80/3678),是最主要的感染型别;其次为HPV16型35例、58型33例,其阳性检出率分别为0.95%(35/3678)、0.90%(33/3678);混合型HPV感染62例,其阳性检出率为1.69%(62/3678);其中HPV43型+X型12例及43型+X型+X型10例、43+X+X+X型1例,占混合型感染的37.10%(23/62),是混合型感染的主要型别;其次是HPV16型的二和三重混合型16例,占混合型感染的25.81%(16/62)。结论 HPV43、16、58型单一型别及43型和16型的混合型是感染南京地区已婚女性宫颈细胞的主要基因型,基因扩增结合基因芯片检测技术可适用于宫颈细胞标本,一次可检测23种HPV感染的基因型,其特异性强,敏感性高,对我国已婚女性宫颈HPV感染基因型的分子流行病学的调查研究具有重要的意义。Objective To research the intribution of 23 genotypes of human papillomavirus（HPV）whieh was well known as the major cause of cervical cancer in human and its clinical significance. Methods Based on the original cell materials from the Depart-ment of Infectious Diseases, Hospital of Yang Zi and the Department of Pathology,Third Affiliated Hospital of Nanjing Traditional Chinese Medical University,23 types of HPV genetype in cervical cell samples from 3 678 cases of married women were tested by PCR and gene-chips technique. Results There were 397 positive cases among 3 678 cervical cell samples of married women. The to-tal HPV infection rates was 10. 79%（397/3 678） ,and the HPV infection rates of single types was 9.11%（335/3 678）. The pre-dominant types from single infection with HPV were in turn as follows：type 43（2.18%,80/3678） ,type 16（0.95% ,35/3 678） ,and type 58 （0.90 %,33/3 678）. The mixed infection rates of HPV was 1.69 % （62/3 678）. The predominant types from mixed infection with HPV were in turn as follows： HPV type 43 superinfection （12 cases）, triple infection （10 cases）, and multiple infection （1 case）. The total infection rate of above three infection modes was 37.10 % （23/62）. Secondly the rate of superinfection and triple in-fection of HPV type 16 was 25.81%（16/62）. Conclusion Single infection of HPV type 43,16 ,and 58,and mixed infection of HPV type 43 and 16 were the main infection modes in 3 678 cases of married women. Gene chip technology could detect multiple HPV genotypes in cervical cell samples with high sensitivity and specificity, which was useful in the pathogenesis and prevention of cervi-cal cancer.

关 键 词：宫颈细胞 人乳头瘤病毒 基因分型 基因芯片 

分 类 号：R737.33[医药卫生—肿瘤]