基于代谢通量分析的SAM和GSH联产溶氧控制策略  

The Dissolved Oxygen Control Strategy Based on Metabolic Flux Analysis for Fermentative Co-Production of S-Adenosylmethionine and Glutathione

作　　者：王大慧[1] 王玉磊[1] 卫功元[1] 邵娜[1] 周捷[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《高校化学工程学报》2013年第1期102-107,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(20906065)

摘　　要：为了提高S-腺苷甲硫氨酸(SAM)和谷胱甘肽(GSH)的联产量,考察了溶氧条件对Candida utilis CCTCC M209298发酵联产SAM和GSH的影响。采用代谢通量分析方法对不同转速下SAM和GSH联产发酵过程中的物质和能量代谢进行分析,确定了较优的溶氧控制策略:前9 h的发酵恒定转速350 r min 1,9 h后控制溶氧不低于30%。在此策略下,C.utilis CCTCC M 209298分批发酵生产SAM和GSH的联产量为520.7 mg L 1。最后分别从发酵动力学、代谢通量分析以及呼吸商变化规律对该溶氧控制策略促进SAM和GSH联合高产的原因进行了定量解释。In order to improve the co-production of SAM and GSH under batch cultivation,the effect of dissolved oxygen（DO） level on fermentative co-production of S-adenosylmethionine（SAM） and glutathione（GSH） in a 5 L stirred fermentor with Candida utilis CCTCC M 209298 was investigated.The metabolic fluxes of intermediates and energy during batch fermentative co-production of SAM and GSH under different agitation rates were calculated by using metabolic flux analysis method.Based on the flux distribution of main intermediates,an optimum DO control strategy of a constant agitation rate of 350 r.min.1 maintained during the first 9 h of fermentation followed with a DO level not less than 30% after 9 h was proposed as an efficient approach for high co-production of SAM and GSH.Under this DO control mode,the product of 520.7 mg.L.1 for batch co-production of SAM and GSH with C.utilis CCTCC M 209298 was achieved.Furthermore,the reason for the improvement of the co-production of SAM and GSH under the proposed strategy was quantitatively interpreted by the fermentative kinetics,metabolic flux analysis and respiratory quotient,respectively.

关 键 词：S-腺苷甲硫氨酸 谷胱甘肽 联产发酵 代谢通量分析 溶氧控制 

分 类 号：TQ464.7[化学工程—制药化工] Q939.97[生物学—微生物学]