16S rDNA克隆文库分析高含盐生物脱硫系统细菌多样性  

Investigation of Bacterial Diversity in the Biological Desulfurization Reactor for Treating High Salinity Wastewater by the 16S rDNA Cloning Method

作　　者：刘卫国[1,2] 梁存珍[2] 杨金生[2,3] 王桂萍[3] 刘苗苗[4] 

机构地区：[1]武汉理工大学资源与环境工程学院,武汉430070 [2]北京石油化工学院环境工程系,北京102617 [3]沈阳理工大学材料科学与工程学院,沈阳110159 [4]中国科学院生态环境研究中心,北京100085

出　　处：《环境科学》2013年第2期767-772,共6页Environmental Science

基　　金：北京市科技新星计划项目(2008B25);北京市属高等学校人才强教深化计划-创新团队-环境治理与调控技术优秀教学团队项目(PHR201107213)

摘　　要：采用分子生物学手段16S rDNA克隆文库方法研究连续运行1 a的生物脱硫反应器中细菌的多样性.从16S rDNA克隆文库中随机挑选40个克隆子进行序列测定(约1 400 bp),对测序结果进行了Blast对比.结果表明,脱硫系统中存在比例较高的优势菌种,有33个克隆子分属于3个不同的细菌类群,1个克隆子属于未知类群,优势细菌类群为Proteobacteria类群(变形菌类群),占85.3%.细菌类群优势顺序为:γ-Proteobacteria类群(55.9%),β-Proteobacteria类群(17.6%),Actinobacteridae类群(8.8%),δ-Proteobacteria(5.9%),α-Proteobacteria(5.9%),Sphingobacteria(2.9%).其中盐生硫杆菌属的Halothiobacillus sp. ST15和硫杆菌属的Thiobacillus sp.UAM-I是系统中的主要脱硫细菌.The bacterial diversity in the biological desulfurization reactor operated continuously for 1 year was studied by the 16S rDNA cloning and sequencing method. Forty clones were randomly selected and their partial 16S rDNA genes （ca. 1 400 bp） were sequenced and blasted. The results indicated that there were dominant bacterias in the biological desulfurization reactor, where 33 clones belonged to 3 different published phyla, while 1 clone belonged to unknown phylum. The dominant bacterial community in the system was Proteobacteria, which accounted for 85.3%. The bacterial community succession was as follows： the γ-Proteobacteria（55.9% ） , β- Proteobacteria（ 17.6% ） , Actinobacteridae （ 8.8% ）, δ-Proteobacteria （ 5.9% ） , α-Proteobaeteria （ 5.9% ）, and Sphingobaeteria （2.9%）. Halothiobacillus sp. ST15 and Thiobacillus sp. UAM-I were the major desulfurization strains.

关 键 词：生物脱硫 16S rDNA 无色硫细菌 高盐含硫废水 细菌多样性 

分 类 号：X172[环境科学与工程—环境科学] X703