月矾中空栓中多指标成分的测定与质量控制  

Determination and quality control of multi-index components in Yuefan Hollow Type Suppository

作　　者：王美[1] 张振秋[1] 袁汀[2] 李妍[1] 梁朔[1] 侯学智[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]中国医科大学附属盛京医院,辽宁沈阳110004

出　　处：《中成药》2013年第2期296-301,共6页Chinese Traditional Patent Medicine

基　　金：辽宁省科技计划项目;月矾中空栓的药效成分基础与药代动力学研究(2009ZZ5010-26)

摘　　要：目的通过定量测定月矾中空栓(儿茶、苦参、青黛、血竭、蛇床子等)中多指标成分,综合评价月矾中空栓的质量。方法采用HPLC法分别测定月矾中空栓中儿茶素、表儿茶素、氧化苦参碱、苦参碱、靛蓝、靛玉红、血竭高氯酸盐,运用综合评分法控制月矾中空栓的质量。结果月矾中空栓中7个成分儿茶素、表儿茶素、氧化苦参碱、苦参碱、靛蓝、靛玉红、血竭高氯酸盐进样量分别在0.115 8～1.158μg(r=0.999 3),0.113 0～1.130μg(r=0.999 1),0.169 0～1.690μg(r=0.999 3),0.269 8～2.698μg(r=0.999 8),0.156 2～1.562μg(r=0.999 7),0.051 80～0.518 0μg(r=0.999 8),0.076 88～0.768 8μg(r=0.999 3)范围内呈良好线性关系。平均回收率(n=5)分别为100.9%,99.9%,100.5%,100.8%,100.9%,99.8%,98.2%。结论本方法能够比较全面的控制月矾中空栓的质量。AIM To evaluate the quality control of Yuefan Hollow Type Suppository （ Catechu, Sophorae flavescentis Radix, Indigo naturalis, Draconis Sanguis, Cnidii Fructus, etc. ） through the determination of multi-index components in the preparation. METHODS The contents of （＋）-catechin, （-）-epicatechin, matrine, oxymatrine, indigo, indirubin and dracorhodin perchlorate in the preparation were determined by HPLC, respectively. The preparation quality was evaluated by comprehensive score method. RESLUTS The calibration curves of （＋）-catechin, （-）-epicatechin, matrine, oxymatrine, indigo, indirubin and dracorhodin＇perchlorate were line- ar in the ranges of 0. 115 8 -1. 158μg （r =0. 999 3）, 0. 113 0 -1. 130 μg （r =0. 999 1）, 0. 169 0- 1. 690 μg （r=0.9993）, 0. 2698 -2.698 μg （r =0.9998）, 0. 1562 - 1.562 μg （r =0.9997）, 0. 051 80 - 0. 518 0μg （ r = 0. 999 8 ） and 0. 076 88 - 0. 768 8 μg （ r = 0. 999 3 ）, respectively. Their average recoveries （n = 5） were 100.9% , 99.9% , 100. 5% , 100. 8% , 100. 9% , 99.8% and 98.2% , respectively. CONCLUSION This method can be used to the quality control of this preparation.

关 键 词：月矾中空栓 儿茶素 表儿茶素 氧化苦参碱 苦参碱 靛蓝 靛玉红 血竭高氯酸盐 定量测定 高效液相色谱法 综合评分 

分 类 号：R927.2[医药卫生—药学]