淤胆大鼠肝内胆管上皮细胞原纤毛形态观察  

Study on Morphological Change of Cholangiocyte Cilia in Extrahepatic Cholestasis Rat Model

作　　者：杨静[1] 杨秀竹[1] 刘洪斌[1] 

机构地区：[1]天津市南开医院中西医结合急腹症研究所,天津300100

出　　处：《中国中西医结合外科杂志》2013年第1期43-45,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市南开医院课题资助(nkyy2008-7)

摘　　要：目的:探讨在大鼠肝外淤胆模型中大胆管和小胆管原纤毛形态的变化。方法:将15只Wistar大鼠随机平分为未处理组(ratsctr)、胆管结扎7d组(ratsL7)和胆管结扎10d组(ratsL10)。用胆总管结扎法制备大鼠肝外淤胆模型。利用扫描电镜技术,比较肝内大、小胆管上皮细胞原纤毛在长度和数量上的异同。结果:ratsctr组大胆管上皮细胞原纤毛平均长度为(7.76±1.14)μm,数量为每视野(10000×)(12±3)根。胆汁淤积后,大胆管上皮细胞原纤毛长度变短:ratsL7为(2.92±1.39)μm,P<0.05;ratsL10缩短为(2.34±0.37)μm,P<0.05,数量增多:ratsL7为(87±12)根,P<0.05;ratsL10为(197±46)根,P<0.05。正常大鼠小胆管上皮细胞原纤毛的平均长度为(5.15±0.89)μm,数量为每视野(4±1)根。胆汁淤积后,小胆管上皮细胞原纤毛长度变短:ratsL7为(2.52±0.96)μm,P<0.05;ratsL10为(2.31±0.53)μm,P<0.05,数量增多:ratsL7为(16±2)根,P<0.05;ratsL10为(15±3)根,P<0.05。结论:结扎胆总管导致肝脏淤胆时,胆管上皮细胞原纤毛数量增多,长度变短。Objective To study the morphology of cholangiocyte cilia in large bile ducts （LBDs） and small bile ducts （SBDs） of rats with extra-hepatic cholestasis induced by bile duct ligation （BDL）. Methods Fifteen rats were divided randomly into three groups （5 in each）. Rats in these groups were subjected to 7-day BDL （rats_L7）, 10-day BDL （rats_L10） and no special treatment （ratsctr）. We characterized the primary cilia of cholangiocytes in these rats using scanning electron microscope. Results In the large bile ducts of ratsctr, the average length and number of primary cilia were （7.76 ± 1.14）Ixm and （12±3）/field, respectively. Compared to the control values, the primary cilia in LBDs were decreased in length （rats_L7 2.92 ± 1.39, P 〈 0.05 and rats_Ll0 2.34 ± 0.37, P 〈 0.05） and increased in numbers （rats_L7 87 ± 12/field, P 〈 0.05 and rats_L10 197 ±46/field, P 〈 0.05） as a result of BDL. In the SBDs of ratsctr, the average length and number of primary cilia were 5.15 ± 0.89 μm and 4 ± 1 /field, respectively. Compared to the control values, the primary cilia in SBDs were decreased in length （rats_L7 2.52 ± 0.96, P 〈 0.05 and rats_L10 2.31 ± 0.53, P 〈 0.05） and increased in numbers （rats_L7 16 ± 2/field, P〈O.05 and rats_L10 15 ± 3 /field, P〈0.05） as a result of BDL. Conclusion After rat BDL, cholangiocyte cilia were increased in number and decreased in length.

关 键 词：肝内胆管上皮细胞 原纤毛 淤胆 扫描电镜 

分 类 号：Q95-33[生物学—动物学] Q241