骨肉瘤中IGF-2基因印迹状态与临床关系的研究  

The Research on the Relationship between IGF-2 Gene Imprinting Status and Clinic Factors in Osteosarcoma

作　　者：张海军 白登彦 袁治国 

机构地区：[1]甘肃省第二人民医院骨科,甘肃兰州730000

出　　处：《实用骨科杂志》2013年第2期132-136,共5页Journal of Practical Orthopaedics

基　　金：甘肃省卫生行业科研项目(GWGL2010-13)

摘　　要：目的探讨骨肉瘤组织中,人胰岛素样生长因子-2(insulin-like growth factors 2,IGF-2)基因的印迹状态及其与各临床病理因素间的关系。方法应用聚合酶链反应、逆转录PCR及限制性内切酶酶切等方法,检测30例骨肉瘤组织中IGF-2基因的印迹状态,分析IGF-2基因印迹丧失(loss of imprinting,LOI)及其与骨肉瘤各临床病理因素之间的关系。结果骨肉瘤组织中IGF-2基因印迹的丧失率为48.5%,瘤近旁组织中IGF-2基因印迹丧失率为21.2%,瘤远旁组织中IGF-2基因印迹丧失率为12.1%。同时,在有肺转移的骨肉瘤病例中IGF-2基因印迹丧失率(60.9%)明显高于无肺转移者(20%,P<0.05)。结论 IGF-2基因印迹丧失是贯穿于骨肉瘤发生、发展全过程的表观遗传异常,其与骨肉瘤的临床及患者预后相关。Objective To investigate the IGF-2 imprinting status in tissues of Osteosarcoma patients and its relationship with the other clinical/pathological parameters in Osteosarcoma. Methods IGF-2 imprinting status was determined by PCR and RT-PCR,followed by restrictive endonuclease （Apa I） digestion in 30 patients with Osteosarco ma. The loss of imprinting （LOI） of IGF-2,and its relationship with the other clinic opathologic parameters was studied in a group of prospectively recruited patients. Results LOI was positive in 5 of 11（48.5 %） of Osteosarcoma tis- sue;2 of 11（21.2% ） in adjacent neighboring tissue ;and 1of 11 （12.1% ） in distant neighboring tissue. The prevalence of IGF-2 LOI in cancer was significantly higher in patients with positive lung metastasis （60. 9%）,as compare to those with negative lung metastasis （20.0%）（P〈0.05）,respectively. Conclusion IGF-2 LOI has been confirmed to be associated with the initiation and development of Osteosarcoma ,which was related with clinical staging and prognosis.

关 键 词：骨肉瘤 人胰岛素样生长因子-2 基因印迹 

分 类 号：R730.2[医药卫生—肿瘤]