罗格列酮对RAW264.7细胞源性泡沫细胞炎症反应及SOCS1和SOCS3表达的影响  

Effects of rosiglitazone on expression of SOCS1/SOCS3 and inflammatory reaction in RAW 264.7 cell-derived foam cells

作　　者：李飞[1] 袁勇[1] 董吁钢[2] 董剑廷[1] 冯力[1] 邓志华[1] 

机构地区：[1]中山市人民医院心血管内科,广东中山528400 [2]中山大学附属第一医院心血管内科,广东广州510080

出　　处：《中国病理生理杂志》2013年第2期261-265,共5页Chinese Journal of Pathophysiology

基　　金：中山市科技计划项目(No.20091A08)

摘　　要：目的:观察过氧化物酶体增殖物激活受体γ(PPARγ)配体罗格列酮是否能够调节泡沫细胞促炎/抗炎反应以及细胞因子信号抑制物1(SOCS1)和细胞因子信号抑制物3(SOCS3)的表达。方法:采用ELISA法测定泡沫细胞培养液中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6和IL-10的水平,并计算TNF-α/IL-10和IL-6/IL-10的比值。采用RT-PCR及Western blotting技术分别观察RAW 264.7细胞源性泡沫细胞SOCS1和SOCS3 mR-NA及蛋白的表达。结果:RAW 264.7细胞源性泡沫细胞组细胞培养液中TNF-α、IL-6和IL-10的水平以及TNF-α/IL-10和IL-6/IL-10的比值均明显高于对照组(control)组。而罗格列酮加入泡沫细胞培养液24 h后,TNF-α、IL-6和IL-10的浓度以及TNF-α/IL-10和IL-6/IL-10的比值均明显低于泡沫细胞组。Control组和泡沫细胞组只有少量SOCS1和SOCS3 mRNA及蛋白的表达,罗格列酮作用于泡沫细胞后SOCS1和SOCS3 mRNA及蛋白的表达均明显高于control组和氧化低密度脂蛋白(ox-LDL)组。结论:罗格列酮上调泡沫细胞SOCS1和SOCS3的表达,抑制泡沫细胞分泌TNF-α、IL-6和IL-10,调节了泡沫细胞促炎/抗炎反应。AIM： To investigate whether perioxisome proliferator-activated receptor y （PPARy） ligand rosiglitazone regulates suppressor of cytokine signaling 1 （ SOCS1 ） and SOCS3 expression as well as pro-inflammatory/anti- inflammatory responses in RAW 264.7 cell-derived foam cells. METHODS ： The concentrations of TNF-a, IL-6 and IL- l0 in the cultured supernatant of RAW 264.7 cell-derived foam cells were detected by ELISA, and the ratios of TNF-ot/IL- 10 and IL-6/IL-10 were calculated. RT-PCR and Western blotting were used to analyze the effects of rosiglitazone on the expression of SOCS1 and SOCS3 at mRNA and protein levels. RESULTS ： The concentrations of TNF-ct, IL-6 and IL-10, and ratios of TNF-cr/IL-10 and IL-6/IL-10 in foam cell group were obviously higher than those in control group, but the concentrations of the above factors in oxidized low-density lipoprotein （ox-LDL） ＋ rosiglitazone group were apparently lower than those in foam cell group. The expression of SOCS1 and SOCS3 at mRNA and protein levels in oxLDL ＋ rosiglitazone group was apparently higher than that in control and foam cell group. CONCLUSION： PPARy ligand rosiglitazone up-reg- ulates the expression of SOCS1 and SOCS3 at mRNA and protein levels and regulates the balance of pro-inflammatory/anti- inflammatory responses in RAW 264.7 cell-derived foam cells.

关 键 词：动脉硬化 炎症 细胞因子类 细胞因子信号抑制物 泡沫细胞 

分 类 号：R363[医药卫生—病理学]