不同强度电针对肥胖大鼠附睾脂肪细胞内质网应激的影响  

Effect of electroacupuncture in different intensities on endoplasmic reti-culum stress in adipose tissues from high-fat diet-induced obese rats

作　　者：郜婕[1] 唐成林[1] 刘仁建[1] 陈晓琳[1] 谢辉[1] 余敏[1] 刘祖丽[1] 

机构地区：[1]重庆医科大学中医药学院,重庆400016

出　　处：《中国病理生理杂志》2013年第2期354-357,共4页Chinese Journal of Pathophysiology

基　　金：重庆市卫生局资助项目(No.07-2-63);重庆市九龙坡区科委资助项目(No.06370302)

摘　　要：目的:通过观察SD肥胖大鼠附睾脂肪组织中脂肪细胞内质网应激-未/折叠蛋白反应(unfolded protein response,UPR)信号转导通路中信号分子p-PERK、CHOP/GADD153、Bcl-2及caspase-12的含量,探索电针对内质网应激的影响。方法:将60只SD大鼠随机分为普食组(n=10)和高脂饮食组(n=50),分别以普通饮食及高脂饮食喂养。10周后选取30只超过普食组大鼠体重20%的高脂饮食组肥胖大鼠进行电针治疗,数字随机分组:高脂饮食组(n=10)、5 mA电针组(n=10)和1 mA电针组(n=10)。高脂饮食组不针刺,电针组针刺双侧足三里(ST 36)和三阴交(SP 6),电针参数选择:疏密波,频率20 Hz,强刺激电针组选用5 mA,弱刺激电针组选用1 mA。2周后治疗结束。用Western blotting检测肥胖大鼠附睾脂肪组织p-PERK和CHOP/GADD153的表达,酶联免疫吸附法(ELISA)检测细胞凋亡相关的Bcl-2及caspase-12的含量。结果:体重:高脂饮食组较普食组增加34.99%,5 mA电针组较普食组增加7.55%,1 mA电针组较普食组增加15.50%(P<0.01或P<0.05);Bcl-2的含量:高脂饮食组较普食组增加20.45%,5mA电针组较普食组增加4.93%,1 mA电针组较普食组增加6.57%,(P<0.01);caspase-12的含量:高脂饮食组较普食组增加53.64%,5 mA电针组较普食组增加7.49%,1 mA电针组较普食组增加24.51%,(P<0.01);p-PERK表达:高脂饮食组较普食组增加251.61%,5 mA电针组较普食组增加61.29%,1 mA电针组较普食组增加145.16%(P<0.01);CHOP/GADD153表达:高脂饮食组较普食组增加438.46%,5 mA电针组较普食组增加26.32%,1 mA电针组较普食组增加184.21%(P<0.01)。结论:电针"足三里"和"三阴交"穴能降低p-PERK、CHOP/GADD153、Bcl-2和caspase-12的含量,5 mA电针组比1 mA电针组效果好。电针能抑制肥胖时机体脂肪细胞的内质网应激反应,本实验结果为电针治疗肥胖炎症状态提供依据。AIM： To investigate the effect of electroacupuncture （EA） in different intensities on unfolded pro- tein response （UPR） signaling pathway in adipose tissues from high-fat diet-induced obese rats. METHODS： Sixty SD rats were randomly divided into 2 groups： common diet group （n = 10） and high-fat diet group （n = 50）. Thirty obese rats in high-fat diet group were chosen and divided into high-fat diet group, 5 mA EA group and 1 mA EA group （ n = 10 each）. EA at frequency of 20 Hz and intensities of 5 mA or 1 mA was applied to ST 36 and SP 6 for 15 rain once daily for 2 weeks. The body weight was detected once a week. The expression of p-PERK and CHOP/GADD153 in epididymal adi- pose tissues was determined by Western blotting. The content of Bcl-2 and caspase-12 in the adipose tissues was measured by ELISA. RESULTS： After EA, the body weight and the expression of p-PERK and CHOP/GADD153 in obese rats were significantly decreased （P 〈 0. 01 or P 〈 0. 05）. The rats in 5 mA EA group exhibited a better improvement on the protein expression than the rats in 1 mA EA group. CONCLUSION： EA has a beneficial regulatory effect on the rats with simple obesity. Moreover, the EA density of 5 mA is superior to 1 mA.

关 键 词：电针 脂肪组织 内质网应激 炎症 

分 类 号：R363[医药卫生—病理学]