运动对急性癫痫大鼠海马神经发生的影响  

Effect of treadmill exercise on neurogenesis in the hippocampus of acute epileptic rats

作　　者：温晓妮[1] 李红[1] 李萍[1] 尤勇 

机构地区：[1]西安体育学院运动医学教研室,陕西西安710068 [2]体育传媒系,陕西西安710068

出　　处：《西安交通大学学报（医学版）》2012年第6期698-701,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省自然科学基金资助项目(No.2011JQ4022)~~

摘　　要：目的探讨中等负荷运动对急性癫痫大鼠海马神经发生的影响。方法应用免疫荧光单标记和激光共聚焦显微镜观察癫痫大鼠在中等负荷运动后海马齿状回新生神经元的5-溴脱氧尿核苷(BrdU)表达情况;应用RT-PCR半定量分析癫痫大鼠在中等负荷运动后海马神经生长因子(neurotrophic growth factor,NGF)的表达情况。结果运动组癫痫大鼠海马齿状回BrdU的表达比未运动组癫痫大鼠表达减少,差异有统计学意义(P<0.01);运动组癫痫大鼠海马NGF基因表达比未运动组癫痫大鼠也下调,差异有统计学意义(P<0.01),且海马NGF基因表达与齿状回BrdU的表达变化相一致。结论中等负荷的运动可减少急性癫痫大鼠海马新生神经元BrdU的表达,而NGF对急性癫痫大鼠神经发生可能有一定的促进作用。Objective To explore the effect of moderate exercise on neurogenesis in the hippocampus of acute epileptic rats.Methods The single-label immunofluorescence with confocal microscope was used to observe the expression of bromodeoxyuridine（BrdU） in the hippocampus of acute epileptic rats after moderate treadmill exercise for 30 min once a day for 7 consecutive days.Semi-quantitative analysis of the expression of nerve growth factor（NGF） gene in the hippocampus of acute epileptic rats was detected by RT-PCR.The correlation between NGF gene expression and BrdU positive cells in the hippocampus was explored.Results The expression of BrdU positive cells in the dentate gyrus of epileptic rats with exercise decreased significantly compared with those without exercise（P0.01）.The expression of hippocampal NGF gene of epileptic rats with exercise was down-regulated compared with those without exercise（P0.01）.The expression pattern of NGF gene in the hippocampus and expression of BrdU positive cells in the dentate gyrus was the same.Conclusion Moderate treadmill exercise can decrease the expression of newborn neuron BrdU in the dentate gyrus of acute epileptic rats.Hippocampal NGF expression may improve the hippocampal neurogenesis of epileptic rats.

关 键 词：运动 海马 神经发生 5-溴脱氧尿核苷 癫痫 神经生长因子 

分 类 号：R742.1[医药卫生—神经病学与精神病学]