大鼠切牙不同程度氟牙症的超微结构观察  

Ultrastructural observation of rat maxillary incisors with varied severity of dental fluorosis

作　　者：邓转云[1] 郭永利[1] 陈明霞[2] 王鹏[2] 

机构地区：[1]西安交通大学医学院口腔医院儿童牙病科,陕西西安710104 [2]基础医学部电镜中心,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2012年第6期783-785,789,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关项目(No.2003K10-G120)~~

摘　　要：目的观察大鼠饮用不同剂量氟水后发生中、重度氟斑牙的下颌切牙超微结构。方法给SD雄性大鼠分别自由饮用0、25、50、100mg/L的含氟水,于不同的时间点用数码相机对大鼠下颌切牙进行唇侧正位照相,将中、重度氟斑牙的左、右侧下颌切牙分别进行扫描电镜及透射电镜的超微结构观察。结果正常鼠切牙的釉质是一层由成釉细胞形成的棕色色素薄层,呈橘黄色、棕黄色;中度氟斑牙表面呈现不同程度棕、白色相间的水平状条纹,透光度降低;重度氟斑牙牙面出现白垩色外观,不透明。扫描电镜显示正常大鼠下颌切牙牙面平整、致密,仅在颈部有小的点窝;中、重度氟斑牙牙面粗糙,点窝在牙冠和牙颈部都明显存在,颈部甚至出现堆积的无釉柱状突起。透射电镜显示随着氟斑牙严重程度加重成釉细胞呈现一系列细胞凋亡的迹象。结论不同程度的氟斑牙分泌期成釉细胞呈现一系列细胞凋亡迹象,且氟斑牙越严重,细胞凋亡的改变越明显。Objective To observe the ultrastructural changes of rat maxillary incisors with varied severity of dental fluorosis induced by different concentration of fluoride in their drinking water.Methods Male SD rats drank water containing graded doses of fluoride（0,25,50 and 100 mg/L）,respectively.At different time points the labial tooth surface of the maxillary incisors in each rat was taken a picture.Each side of the maxillary incisors with moderate or severe fluorosis was observed under scanning electron microscope（SEM） and transmission electron microscope,respectively.Results The enamel of rat normal incisors,of an orange-yellow or brown-yellow color,was a film of brown pigment layer formed by ameloblasts.Enamel changes in the rat incisor teeth with fluorosis occurred as brown and white horizontal marks（moderate fluorosis） and progressively developed into a white chalky appearance（severe fluorosis）.SEM showed the surface of teeth with severe fluorosis was mainly rough with discrete pits and small parts of relatively sound enamel.The subsurface enamel of teeth with severe fluorosis had irregular cross-sectional shape of rod and more extensive inter-rod enamel spaces in comparison with other parts of enamel.The surface enamel of teeth with very mild fluorosis was mainly smooth,similar to the sound enamel,with small parts of rough enamel.In subsurface enamel of teeth with very mild fluorosis,no difference was found between cross-sectional shape of rod and dimension of inter-rod enamel spaces in comparison with other parts of enamel.Conclusion Secretory ameloblasts with varied degrees of dental fluorosis showed a series of signs of apoptosis.The more severe dental fluorosis is,the more obvious changes in apoptosisare.

关 键 词：氟斑牙 大鼠切牙 严重程度 超微结构 

分 类 号：R781[医药卫生—口腔医学]