松弛素对大鼠蛛网膜下腔出血后转化生长因-β1和蛛网膜纤维化作用的研究  

Effects of Relaxin on TGF-β1 and Arachnoid Fibrosis in Rats with Subarachnoid Hemorrhage

作　　者：严雯[1] 冯敏[2] 赵洪洋[2] 廖完敏[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院综合科,武汉430030 [2]华中科技大学同济医学院附属协和医院神经外科,武汉430022 [3]华中科技大学同济医学院附属同济医院麻醉科,武汉430030

出　　处：《神经损伤与功能重建》2013年第1期23-25,共3页Neural Injury and Functional Reconstruction

基　　金：高等学校博士学科点专项科研基金(新教师基金)(No.20100142120043)

摘　　要：目的:研究松弛素(RLX)对大鼠蛛网膜下腔出血(SAH)后脑脊液中转化生长因子-β1(TGF-β1)的作用及其与蛛网膜纤维化的相关性。方法:SD大鼠60只,随机分为空白组、模型组、干预组各20只。采用枕大池两次注血法建立大鼠SAH模型,空白组仅抽取脑脊液,模型组和干预组制备SAH模型,干预组在建模后的第2天开始鞘内注射RLX。在不同时间点检测脑脊液中TGF-β1的浓度,应用图像采集分析系统测量胶原纤维含量。结果:干预组中脑脊液中TGF-β1的浓度较模型组显著降低(P<0.05)。Masson染色显示模型组蛛网膜下腔纤维含量明显增多,与干预组和空白组相比有统计学差异(P<0.05),干预组蛛网膜下腔纤维含量较空白组增多,较模型组减少,差异均具有统计学意义(P<0.05)。结论:蛛网膜下腔内注入RLX可降低SAH后脑脊液中TGF-β1浓度,具有一定的抗蛛网膜纤维化作用。Objective： To evaluate effects of relaxin （RLX） on transforming growth factor-β1 （TGF-β1） and arachnoid fibrosis in rats model of subarachnoid hemorrhage（SAH）. Methods： Sixty SD rats were randomly divided into groups blank, model and RLX with 20 rats in each group. SAH models were established by twice injections of blood into cistern magna. The blank group was extracted cerebrospinal fluid （CSF）, SAH models were established in the model and RLX groups. RLX was infused into subarachnoid space via foramen magnum of rats in RLX group. TGF-13 1 in CSF was measured at different times, and the collagen fibers were analyzed by micrograph collection and analysis system respectively. Results： The TGF-β1 in cerebrospinal fluid in the RLX group was significantly lower than that in the model group （P〈0.05）.Masson staining showed that the fiber content in the model group was increased significantly, when compared with the RLX and blank groups. The subarachnoid fiber content in the RLX group was significantly increased relative to that in the blank group, but reduced compared to the model （P〈0.05）. Conclusion： RLX can reduce TGF-β1 concentration in CSF in rats with SAH, and in turn easing arachnoid fibrosis.

关 键 词：松弛素 蛛网膜下腔出血 纤维化 转化生长因子-Β1 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]