硒元素对急性癫痫小鼠脑组织氧化损伤的影响  

Effect of selenium on oxidation damages to the brains of mice with acute epilepsy seizures

作　　者：王禹[1] 那猛[1] 朱建[1] 林志国[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经外科,哈尔滨150001

出　　处：《中国临床神经外科杂志》2013年第2期79-81,共3页Chinese Journal of Clinical Neurosurgery

基　　金：国家自然科学基金项目(编号:81071215);黑龙江省自然科学基金项目(编号:D201062);黑龙江省教育厅科研项目(编号:10551186)

摘　　要：目的探讨微量元素硒对小鼠急性癫痫发作后脑组织氧化损伤的影响。方法将40只健康昆明小鼠按体重随机分为3组,即实验组、阳性对照组和阴性对照组。实验组小鼠经口灌胃Na2SeO2(20μg/kg),阳性对照组和阴性对照组小鼠给予0.9%氯化钠溶液,1次/d,连续7d;给药后第8天实验组和阳性对照组小鼠腹腔内注射青霉素600万U/kg,阴性对照组腹腔注射等体积0.9%氯化钠溶液,观察小鼠癫痫发生时的行为改变;小鼠被完全点燃后处死,比色法测定小鼠脑组织中谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。结果腹腔内注射青霉素后,实验组小鼠癫痫样发作出现时间较阳性对照组晚,且发作级别低。与阴性对照组相比,阳性对照组脑组织中GSH-Px活性显著下降(P<0.01),MDA含量显著上升(P<0.01);与阳性对照组相比,实验组GSH-Px活性显著升高(P<0.01),MDA含量显著下降(P<0.01)。结论急性癫痫可引起脑组织氧化损伤,损害神经细胞。补硒可增加脑组织中GSH-Px活性,从而减轻氧自由基对机体造成的损害。Objective To investigate the effect of selenium（Se）on oxidation damages to the brains of mice with acute epilepsy seizures. Methods Forty healthy mice were randomly divided into three groups, i.e. positive control group （n=15）, negative control group （n=10） and experimental group （n=15）. The oral administration of Na2SeO2 （20 μg/kg） to the mice was performed once a day for 7 days in the experimental group. The oral administration of the physiological saline of the same volume to the mice was performed once a day for 7 days in both the control groups. The epilepsy was induced by intraperitoneal injection of penicillin （6 000 000 IU/Kg） in the experimental and positive control groups and the physiological saline of the same volume was intraperitoneally injected in the negative control group the eighth day after the oral administration of the agents. The behavioral change in the mice were observed in all the groups. All the mice were killed after the complete seizures, and the activity of glutathione peroxidase（GSH-Px） and contents of malondialdehyde（MDA） in the brain were measured by chemical colorimetric analysis in all the groups. Results The onset of acute epilepsy in the mice was delayed and the symptom was mild after the intraperitoneal injection of penicillin in the experimental group compared with the positive control group. GSH-Px activity was significantly lower and the contents of MDA was significantly higher in the cerebral tissues in the positive group than those in the negative and experimental groups （P〈0.01）. Conclusions Acute epilepsy may produce the oxidation damages to the cerebral neurons which may be delayed and relieved by oral administration of Na2SeO2 in the mice. Its mechanism may be related to the increase in the activity of GSH-Px and decrease in the content of MDA in the cerebral tissues induced by Se in the mice with epilepsy.

关 键 词：硒 急性癫痫 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：R742.1[医药卫生—神经病学与精神病学]