诱捕受体3在宫颈癌中的表达及临床意义  

Expression and clinical significance of DcR3 in cervical cancer

作　　者：王治荣[1] 邢兰瑛[1] 刘宁侠[1] 王笑笑[1] 冯敏娟[1] 

机构地区：[1]西安交通大学医学院第二附属医院妇产科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2013年第2期224-228,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省社会发展攻关计划资助项目(No.S2012SF3118)~~

摘　　要：目的探讨诱捕受体3(DcR3)表达与宫颈癌发生、发展的关系及其可能机制,为宫颈癌的诊治提供新的理论线索。方法应用ELISA法检测宫颈癌、宫颈上皮内瘤变(CIN)、健康女性外周血清及宫颈癌患者术前、术后1周配对组外周血清中DcR3的表达水平;应用免疫组织化学(SP)法检测宫颈癌(从上述血清宫颈癌组随机抽取组织)、CIN及正常宫颈组织中DcR3的表达。结果 ELISA检测显示,宫颈癌组DcR3血清水平显著高于其他两个对照组(P<0.05),其表达水平在健康女性组、CIN组、宫颈癌组间呈逐渐升高趋势;宫颈癌组术后1周血清DcR3较术前显著降低(P<0.05)。免疫组化显示,宫颈癌组DcR3表达阳性率为72.5%,CIN、正常宫颈的表达阳性率分别为45%、6.7%(P<0.05);宫颈癌组DcR3血清水平及组织表达阳性率均随着临床分期、淋巴结转移及分化程度加重而升高(P<0.05);宫颈癌组DcR3血清水平及组织表达阳性率之间呈正相关(P<0.05)。结论 DcR3过度表达可能在宫颈癌的发生、发展中起着重要作用;血清学检测DcR3水平可以作为宫颈癌筛查、诊断及治疗方案选择的一种辅助手段。Objective To investigate the relationship of death decoy receptor 3 （DcR3） expression with the pathogenesis and development of cervical cancer so as to provide a new theoretical clue for diagnosis and treatment of cervical cancer. Methods Serum concentrations of DcR3 were measured by enzyme-linked immunosorbent assay （ELISA） in healthy women, patients with cervical intraepithelial neoplasia （CIN） and patients with cervical cancer, one week before and after surgery. The immunohistochemical streptavidin-peroxidase method （SP） was employed to detect the expression of DcR3 in cervical cancer, CIN and normal tissues. Results ELISA showed that serum concentrations of DcR3 were significantly higher in cervical cancer than in the two control groups （P〈005）. Concentrations of DcR3 increased gradually in healthy women, CIN and cervical cancer groups. One week after operation, serum concentrations of DcR3 decreased significantly in the corresponding cervical cancer groups compared with the level before operation （P〈0.05）. Immunohistochemistry showed the positive expression of DcR3 was 72.5%, 45% and 6. 7% in cervical cancer, CIN and normal tissues, respectively （P〈0. 05）. The expression of DcR3 in serum and tissues was found to be positively correlated with clinicopathologic parameters of cervical cancer （P〈0.05）. The serum concentration had a positive correlation with DcR3 expression in cervical cancer tissues （P 〈 0. 05）. Oonclusien The overexpressed DcR3 in cervical cancer may contribute to the pathogenesis and development of the cancer. Serological monitoring of DcR3 protein may be useful for screening, diagnosis and treatment of cervical cancer.

关 键 词：宫颈癌 诱捕受体3(DcR3) 酶联免疫吸附试验(ELISA) 免疫组织化学 

分 类 号：R730[医药卫生—肿瘤]