吉西他滨作用于肝癌HepG2细胞后对DR5、caspase-8、caspase-9和caspase-3表达的影响  

Expressions of DR5,caspase-8,caspase-9 and caspase-3 in HepG2 Cell Line Treated with Gemcitabine

作　　者：聂艳丽[1] 阮之平[2] 南克俊[2] 

机构地区：[1]湖北省肿瘤医院腹部肿瘤内科,武汉430079 [2]西安交通大学第一附属医院肿瘤内科

出　　处：《肿瘤防治研究》2013年第2期134-137,共4页Cancer Research on Prevention and Treatment

摘　　要：目的研究吉西他滨作用于HepG2细胞后DR5、caspase-8、caspase-9、caspase-3的表达,以期探讨吉西他滨诱导HepG2细胞凋亡的机制。方法应用MTT法和流式细胞仪体外研究吉西他滨对HepG2细胞增殖的影响;采用免疫细胞化学及Western blot法,观察吉西他滨作用于HepG2细胞24h后DR5、caspase-8、caspase-9、caspase-3的表达情况。结果吉西他滨对肝癌HepG2细胞的抑制作用呈时间和浓度依赖性;吉西他滨作用于HepG2细胞24h后,细胞表面DR5及caspase-8、caspase-9、caspase-3的表达均增强。结论吉西他滨诱导肝癌HepG2细胞凋亡的机制可能与其能够上调DR5及caspase-8、caspase-9、caspase-3的表达有关。Objective To research DR5,caspase-8,caspase-9 and caspase-3 expressions in HepG2 cells treated by gemcitabine,and preliminary apoptosis mechanism induced by gemcitabine.Methods After treated by virous cencetrations,HepG2 cell growth inhibition and apoptosis was assessed by MTT and FMC respectively;the expressions of DR5,caspase-8,caspase-9 and caspase-3 were detected by immunohistochemical method and Western blot.Results Gemcitabine apparently inhibited the growth of HepG2 and induced apoptosis with a time-dependent and concentration-dependent way After treated by gemcitabine for 24 h,the expressions of DR5,caspase-8,caspase-9 and caspase-3 were up-regulated.Conclusion Gemcitabine induced the apoptosis of HepG2 through up-relation of DR5,caspase-8,caspase-9 and caspase-3.

关 键 词：吉西他滨 HepG2细胞 DR5 CASPASE-8 CASPASE-9 caspase-3 

分 类 号：R735.7[医药卫生—肿瘤]