花姜酮对胰腺癌细胞迁移和侵袭的影响及机制的研究  

Effect of zerumbone on migration and invasion of human pancreatic cancer cell lines

作　　者：刘婷婷[1] 王琪[1] 舒时珍[1] 蒋荷[1] 姚静静[1] 王立夫[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化内科,上海200025

出　　处：《内科理论与实践》2013年第1期15-19,共5页Journal of Internal Medicine Concepts & Practice

基　　金：国家自然科学基金(项目编号:30971130);国家自然科学基金(项目编号:81272263);上海市科学技术委员会科研计划项目(项目编号:11JC1407601)

摘　　要：目的:探讨花姜酮(zerumbone)对人胰腺癌细胞株BxPC-3和Panc-1迁移和侵袭的影响及其相关基因的表达。方法:以BxPC-3和Panc-1为研究对象,通过四甲基偶氮唑盐(MTT)实验选择毒性较小的花姜酮浓度,用细胞划痕实验、Transwell小室迁移和侵袭实验检测药物对细胞迁移和侵袭能力的影响,蛋白质印迹(Western blot)检测各细胞组CXC族趋化因子受体4(CXCR4)、促分裂原活化蛋白酶激酶1/2(MEK1/2)、磷酸化MEK 1/2(p-MEK1/2)、细胞外调节激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)蛋白表达变化。结果:花姜酮对BxPC-3和Panc-1细胞的生长均有抑制作用,且随着药物浓度的增高和作用时间的延长而增强(P<0.05)。低浓度药物作用后BxPC-3和Panc-1的划痕迁移率都低于对照组(P<0.05)。与对照组相比,Transwell迁移和侵袭实验中BxPC-3和Panc-1用药组的平均穿膜细胞数均减少(P<0.05)。蛋白质印迹检测结果表明:花姜酮抑制CXCR4、p-MEK1/2、p-ERK1/2蛋白的表达。结论:花姜酮具有抑制胰腺癌细胞株BxPC-3和Panc-1迁移和侵袭的作用,可能与下调CXCR4、p-MEK1/2、p-ERK1/2表达水平有关,为肿瘤的靶向治疗提供新的依据。Objective To investigate the effect of zernmbone on migration, invasion and related gene expression of human pancreatic cancer cell lines BxPC-3 and Panc-1. Methods Human pancreatic cancer cell lines BxPC-3 and Panc-1 were selected as the object for investigation. Methyl thiazolyl tetrazolium （MTF） assay was performed for choosing the appropriate concentration of zernmbone. Cell scratch test, transwell migration assay and invasion experiment were performed to assess the effect of zerumbone on cell migration and invasion. The expression levels of CXC chemokine receptor 4 （CXCR4）, mitogen-activated protein kinase （MEK）I/2, extracellular-signal-regulated kinase （ERK）I/2, phosphorylated MEK1/2 （p-MEK1/2）, phosphorylated ERK1/2 （p-ERK1/2） were measured by Western blot. Results Zerumbone could inhibit the proliferation of BxPC-3 and Panc-1 cells in a dose- and time-dependent manner （P〈0.05）. Cell scratch test showed that the migration rates of BxPC-3 and Panc-1 cell after zernmbone treatment were lower than those of the control group （P〈0.05）. The average number of migration and invasive cells was fewer in zerumbone treatment group than that in control group （P〈0.05）. Western blot assay showed that zerumbone reduced the expression of CXCR4, p-MEK1/2 and p- ERK1/2. Conclusions Zerumbone could inhibit the migration and invasion of human pancreatic cancer cell lines BxPC-3 and Panc-1. This might be related to the down-regulation of expression of CXCR4, p-MEK1/2, p-ERK1/2, and this may provide a new insight for the tumor targeted therapy.

关 键 词：花姜酮 胰腺癌细胞 细胞迁移和侵袭 CXC族趋化因子受体4 促分裂原活化蛋白酶激酶1 2 

分 类 号：R735.9[医药卫生—肿瘤]