高效液相色谱紫外检测法测定茶(药)用菊花的绿原酸含量  

Determination of chlorogenic acid in tea(medicine)chrysanthemum samples by HPLC and ultraviolet detector

作　　者：杨小秋[1] 韩磊[1] 傅厚暾[1] 蒋细旺[2] 

机构地区：[1]江汉大学化学与环境工程学院,武汉430056 [2]江汉大学生命科学学院,武汉430056

出　　处：《分析仪器》2013年第1期12-15,共4页Analytical Instrumentation

基　　金：武汉市科技攻关课题"茶用菊花耐热种质创新和推广应用"(200851799524-02);武汉市重大科技创新专项"菊花耐热体细胞无性系变异研究"

摘　　要：建立了高效液相色谱分离以及四波长紫外检测测定菊花中绿原酸的方法。以甲醇:磷酸二氢钠缓冲溶液(浓度为0.1mol/L)=40∶60(用磷酸调节pH值为2.7)为淋洗液进行分离,对230nm、254nm、275nm、327nm4个波长检测出的绿原酸信号加以比较进行定性定量。用该方法对菊花样品中的绿原酸进行测定,检出限为0.085mg/L,精密度低于5%,结果令人满意。实验证明,该方法准确、可行,可以很好地对菊花中的绿原酸进行定性定量研究。To identify the chlorogenic acid in chrysanthemum sample, a method of high per- formance liquid chromatography and four wavelengths ultraviolet detector was developed. The chrologeniei acid was carried out from chrysanthemum samples with soxhlet extraction, then was separated in HPLC with the mobile phase of methanol--0.1mol/L sodium dihydrogen phosphate buffer, the proportion of the solute is 40：60 and the buffer pH was 2.7. The signals of chloro- genie acid in the wavelength of 230nm, 254nm, 275nm, 327nm were compared, and the comparison results and the retention time were similar basically, so the chlorogenic acid was identified effec- tively and the best detect wavelength was chosen. The method was used in the detection of chlo- rogenic acid of chrysanthemum samples, the detection limit was 0. 085 mg/L and the RSD was lower than 5 %, and the added recovery was satisfactory.

关 键 词：高效液相色谱 紫外检测 菊花 绿原酸 

分 类 号：TS272.7[农业科学—茶叶生产加工] O657.72[轻工技术与工程—农产品加工及贮藏工程]