不同时间低氧条件对人胃黏膜上皮细胞凋亡的影响  

Effects of apoptosis on human gastric epithelial cell strain-1 of hypoxia at different time

作　　者：张晓岩[1] 杨生玺[1] 李玉琴[2] 蒋虹[2] 侯成荣[1] 耿排力[1] 

机构地区：[1]青海大学医学院基础医学部,西宁810001 [2]青海大学附属医院

出　　处：《山东医药》2012年第48期26-28,I0003,共4页Shandong Medical Journal

基　　金：青海省科技厅应用基础研究项目(2010-Z-727)

摘　　要：目的研究低氧条件对体外培养人胃黏膜上皮细胞株GES-1凋亡的影响。方法建立GES-1细胞体外低氧培养模型。在低氧处理后不同时间点(24 h、48 h、72 h)MTT法检测存活率、电镜观察形态变化、TUNEL染色观察细胞凋亡。结果低氧条件作用24 h、48 h、72 h后,GES-1细胞存活率均下降(P<0.05),细胞凋亡指数均升高(P<0.05),透射电镜均观察到部分凋亡细胞。结论低氧条件可明显抑制GES-1细胞生长并诱导其凋亡。Objective To investigate the effect of hypoxia on apoptosis in human gastric epithelial cell strain-1 （ GES- 1 ）. Methods Hypoxia culture model in vitro was established to test the apoptosis in GES-1 cell line. After GES-1 was cultured in hypoxia circumstance for a given period of time （24 h, 48 h, 72 h）. The survival rate of cell and apoptosis were measured by MTI＇, electromicroscope, TUNEL staining. Results The survival rate of GES-1 was decreased （P 〈 0.05 ）, but the apoptosis index was increased （P 〈 0.05 ） in hypoxia at 24 h, 48 h, 72 h. The transmission electron microscope showed some apoptosis-like morphologic changes. Conclusion Hypoxia has an obvious inhibitory effect on the cell proliferation, and increases apoptosis of GES-1.

关 键 词：低氧 胃黏膜上皮细胞 凋亡 

分 类 号：R363.1[医药卫生—病理学]