不同蛋白质供给水平对肝硬化大鼠血清细胞因子的影响  

Impact of different levels of protein supply on the cytokines in cirrhotic rats

作　　者：王真真[1,2] 齐玉梅[3] 郝春满[1] 陈亚军[3] 肖慧娟[3] 徐常杰[1] 

机构地区：[1]天津医科大学三中心临床学院,天津300170 [2]天津市公安医院 [3]天津市第三中心医院

出　　处：《山东医药》2012年第48期32-34,I0003,共4页Shandong Medical Journal

基　　金：天津市卫生局科技攻关项目(10KG106)

摘　　要：目的探讨不同蛋白质供给水平对肝硬化大鼠血清细胞因子的影响。方法将清洁级健康雄性Wistar大鼠60只随机分为4组,即正常对照组(N组)、低蛋白组(L组)、标准蛋白组(S组)、高蛋白组(H组)。采用CCl4和酒精复合法复制肝硬化大鼠模型。成模后采用含不同蛋白质水平的饲料进行干预,干预周期为6周。在干预前后取血检测各组大鼠血清TNF-α、IL-10、IGF-1水平。结果与干预前比较,干预后各模型组大鼠的TNF-α和IGF-1均升高(P<0.05);干预后,L组TNF-α低于H组(P<0.05),S组IL-10高于H组(P<0.05),S组IGF-1高于L组(P<0.05)。结论不同蛋白质供给水平对肝硬化大鼠血清细胞因子均有所改善;正常水平的蛋白质供给即可改善免疫功能,而高蛋白的摄入并不能增强这种作用。Objective To explore the effect of different levels of protein on the cytokines in rats with liver cirrhosis.Methods A total of 60 healthy male Wistar rats were randomly divided into four groups： normal control group（N）,low-protein group（L）,the standard protein group（S）,high-protein group（H）.Cirrhotic rat model was established by CCl4 and alcohol.After modeling,supply different levels of protein to relevant groups,the intervention period was six weeks.Before and after the intervention,the serum levels of TNF-α,IL-10 and IGF-1 of rats in each group were detected.Results After intervention,the TNF-α and IGF-1 of model rats were significantly increased（P〈0.05）;but TNF-α of L group was lower than that in H group（P〈0.05）.The level of IL-10 in S group was higher than that in H group（P〈0.05）,and IGF-1 of S group was higher than that in L group（P〈0.05）.Conclusion After the supply of different levels of protein to cirrhotic rats,the levels of cytokines had improved;normal protein supply could improve immune function,but high protein intake didn′t enhance this effect.

关 键 词：蛋白质 肝硬化 细胞因子 

分 类 号：R575.2[医药卫生—消化系统]