紫外分光光度法测定刺梨总三萜与总黄酮含量  

The content determination of total triterpenoids and flavonoids in Rosa roxbughii by ultraviolet spectrophotometry

作　　者：南莹[1,2] 李齐激[2] 马琳[2] 张倩茹[1] 杨小生 

机构地区：[1]遵义医学院,贵州遵义563099 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550001

出　　处：《遵义医学院学报》2012年第6期473-476,共4页Journal of Zunyi Medical University

基　　金：国家自然科学基金项目(NO:30973620);贵州省科技厅基金项目(NO:黔科合SY字[2011]3004)

摘　　要：目的测定贵州野生和栽培刺梨总三萜与总黄酮的含量。方法采用比色法测定刺梨中总三萜与总黄酮的含量。结果野生和栽培刺梨鲜果、粗粉总三萜含量分别为2.578%、16.990%、1.129%、12.485%。野生和栽培刺梨鲜果、粗粉总黄酮含量分别为0.088%、0.746%、0.077%、0.721%。结论利用比色法测定刺梨总三萜的含量,该方法操作简便、快速、稳定,可以用于刺梨的质量控制。Objective To determinate the content of total triterpenoids and flavonoids in Rosa roxbugh- ii. Methods The content of total triterpenoids and flavonoids in Rosa roxbughii were determinated by col- orimetric method. Results The contents of total triterpenoids in the wild type and cultivated type of Rosa roxbughii were 2. 578%, 16. 990%, 1. 129% and 12. 485%, respectively. The contents of total fla- vonoids in the wild type and cultivated type of Rosa roxbughii were 0. 088%, 0.746%, 0. 077% and 0. 721%, respectively. Conclusion The content of total triterpenoids in Rosa roxbughii was determined for the first time by colorimetric method and this method is easy, rapid and stabilization and might be used for monitoring the quality of Rosa roxbughii.

关 键 词：刺梨 比色法 总三萜 总黄酮 

分 类 号：R284.1[医药卫生—中药学]