非小细胞肺癌患者化疗前后外周血T淋巴细胞亚群的变化  

Analysis on the Change of Peripheral Blood T Lymphocyte Subsets before and after Chemotherapy in Patients with Non-Small Cell Lung Cancer

作　　者：张锦英[1] 朱陵君[1] 卢凯华[1] 殷咏梅[1] 郭人花[1] 顾艳宏[1] 刘平[1] 束永前[1] 

机构地区：[1]南京医科大学第一附属医院肿瘤生物治疗中心,江苏南京210029

出　　处：《现代生物医学进展》2012年第35期6814-6818,共5页Progress in Modern Biomedicine

基　　金：江苏省卫生厅社会发展基金(P200965)

摘　　要：目的:探讨非小细胞肺癌(NSCLC)患者细胞化疗前后T淋巴细胞亚群的变化及其意义。方法:采用流式细胞术检测NSCLC患者及健康对照者外周血中CD3+、CD4+、CD8+、CD4+/CD8+及NK细胞的比例。结果:与对照组比较,NSCLC患者化疗前及化疗后CD3+、CD4+、CD4+/CD8+、NK细胞含量均显著降低(P<0.01);化疗后CD3+、CD4+、CD4+/CD8+、NK细胞比例与化疗前比较均显著升高(P<0.05);化疗前后CD8+细胞比例无显著变化(P>0.05)。治疗后疾病控制组(DCR)患者的CD3+、CD4+、CD4+/CD8+及NK细胞均显著高于治疗后进展(PD)组患者组(P<0.05);CD8+细胞的比例无显著性变化(P>0.05)。结论:NSCLC患者细胞免疫功能低下,通过流式细胞术检测患者外周血T淋巴细胞亚群及NK细胞的变化对评估患者的细胞免疫功能及肿瘤化疗疗效具有重要的临床意义。Objective： To study the change of peripheral blood T lymphocyte subsets before and after chemotherapy and its clinical significance in patients with non-small cell lung cancer.Methods： To determine the CD3＋,CD4＋,CD8＋,CD4＋/CD8＋and NK cell in peripheral blood of non-small cell lung cancer and healthy adult by flow cytometry.Results： The level of CD3＋,CD4＋and CD4＋/CD8＋ ratio and NK cell before and after chemotherapy in NSCLC were decreased significantly in comparison with control group（P0.01）,and the level of CD3＋,CD4＋ and CD4＋/CD8＋ ratio and NK cell after chemotherapy were increased significantly in comparison with before chemotherapy in NSCLC（P0.05）,while CD8＋cells had no significant change（P0.05）.The level of CD3＋,CD4＋and CD4＋/CD8＋ratio and NK cell in DCR group were increased significantly in comparison with PD group（P0.05）,while CD8＋ cells had no significant change（P0.05）.Conclusion： The cellular immune function of the NSCLC patients was lowered.It has important clinical significance to detecting the change of T lymphocyte subgroup and NK cells by flow cytometry to assess the immune function and the efficacy of chemotherapy in patients with NSCLC.

关 键 词：非小细胞肺癌 淋巴细胞亚群 流式细胞术 细胞免疫 肿瘤化疗 

分 类 号：R734.2[医药卫生—肿瘤]