BMP-7对大鼠脑缺血损伤后神经细胞凋亡的影响  

Effect of BMP-7 on the Nerve Cell Apoptosis Induced by Ischemia and Eperfusion in Rats

作　　者：于竹芹[1] 鲁成龙[2] 李红云[2] 裴海涛[2] 

机构地区：[1]青岛大学医学院附属松山医院神经科,山东青岛266021 [2]青岛大学医学院附属医院急诊神经科,山东青岛266003

出　　处：《临床医学工程》2013年第2期156-158,共3页Clinical Medicine & Engineering

基　　金：山东省自然科学基金资助项目(NoZR2009CM121)

摘　　要：目的观察骨形态发生蛋白-7(BMP-7)对大鼠局灶性脑缺血/再灌注损伤诱导的神经细胞凋亡与凋亡相关蛋白Caspases-3和Bcl-xl表达的影响。方法成年健康雄性Sprague-Dawley大鼠53只,应用线栓法建立大鼠局灶性脑缺血再灌注损伤模型,随机分为假手术组、模型组、治疗组,治疗组于再灌注后立即给予尾静脉注射BMP-7(0.1mg·kg-1),假手术组和模型组同步给予等量生理盐水,再灌注24h后进行神经功能缺陷评分,原位末端标记(TUNEL)法检测梗死脑组织细胞凋亡,免疫组织化学法及Western Blot法检测Caspase-3和Bcl-xl的表达。结果缺血再灌注24h后,与模型组比较,BMP-7治疗组大鼠神经功能缺陷评分明显降低(P<0.01),TUNEL检测阳性细胞数明显减少(P<0.01),Western Blot显示缺血侧脑组织Caspase-3表达明显减弱(P<0.01),Bcl-xl表达明显增强(P<0.01)。结论 BMP-7可通过调节Caspase-3和Bcl-xl表达,抑制神经细胞凋亡,发挥神经保护作用。Objective To observe the effect of bone morphogenetic protein-7 （BMP-7） on the nerve cell apoptosis and expression of apoptosis related proteins Caspase-3 and Bcl-xl aider focal cerebral ischemia-reperfusion injury in rats. Methods We used 53 adult healthy male SD rats and established as middle cerebral ischemia-reperfusion （I/R） injury model by thread tying method. The rats were equally randomized into the sham-operation group, model group and BMP-7 group. The rats in BMP-7 group were immediately intervened with BMP-7 （0.1 nag.kg-＇） via caudal vein injection after IJR injury, while the rats in sham-operation group and model group were injected with equal volume of saline. After reperfusion 24 hours, the rats＇ neurological deficit scores were recorded, the apoptotic cells were counted by TUNEL assay, immunohistochemistry stain and Western Blot methods were used to determine the expression of Caspase-3 and Bcl-xl. Results As compared with model group after I/R 24 hours, the rats＇ neurological deficit scores in the BMP-7 group were significantly lower （P〈0.01）, the apoptosis cells detected by TUNEL were significantly fewer（P〈0.01）, and the expression of Bcl-xl in the ischemic side was significantly enhanced（P〈0.01）, but Caspase-3 significantly decreased （P〈0.01）. Conclusion BMP-7 provides protective effect on cerebral ischemia-reperfusion injury with inhibiting nerve cell apoptosis by up-regulating Bcl-xl and down regulating Caspase-3 expression.

关 键 词：BMP-7 脑缺血再灌注损伤 细胞凋亡 CASPASE-3 Bcl—xl 

分 类 号：R741[医药卫生—神经病学与精神病学]