超高效液相色谱-串联质谱法快速检测水产品中喹乙醇及其代谢物3-甲基-喹噁啉-2-羟酸的残留  

Rapid Determination of the Residues of Olaquindox and 3-methylquinoline-2-carboxylic Acid in Aquatic Products by UPLC-MS/MS

作　　者：薛良辰[1,2] 彭玉芬[2] 刘陆[2] 蔡勤仁[2] 冯家望[2] 余以刚[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]珠海出入境检验检疫局技术中心,广东珠海519015

出　　处：《现代食品科技》2013年第2期413-415,共3页Modern Food Science and Technology

摘　　要：建立了水产品中喹乙醇（OQX）及其代谢物3-甲基-喹噁啉-2-羟酸（MQCA）的超高效液相色谱串联质谱快速测定方法。试样经乙酸乙酯＋乙腈（1＋1，V／V）液液萃取提取和净化后，BEHC18UPLC进行分离，多反应选择离子检测。方法的回收率和变异系数分别在62．5％91．4％和2．6％-11．8％之间。本方法对水产品中喹乙醇和3-甲基-喹噁啉-2-羟酸的检测限均为1．0~g／kg。This study presented a ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS） method for fast determination of the residues of olaquindox and 3-methylquinoline-2-carboxylic acid in aquatic products. Resident of olaquindox and 3-methylquinoline-2-carboxylic acid ware extracted by the liquid- liquid method using ethyl acetate＋ acetonitrile （ 1＋1, V/V）.After BEH C 18 LC gradient elution separation,the analytes ware determined under multi-reaction monitoring （MRM） scan type with tandemmass analyzer using positive polarity mode. The recoveries are in the range of 62.5%--91.4%, the relative standard deviation are in the range of 2.6%11.8%. The limit of detection （LOD） for olaquindox and methyl-3-quinoxaline-2-carboxylic acid in aquatic products was 1.0 μg/kg.

关 键 词：超高效液相色谱串联质谱 喹乙醇 3-甲基-喹噁啉-2-羟酸 残留 

分 类 号：R155.5[医药卫生—营养与食品卫生学]