气滞血瘀证大鼠NO/NOS体系的变化  

Change of NO/NOS in Rats of Qi-blood Stagnation

作　　者：宁天一[1] 王婷婷[1] 姜静[1] 程嘉艺[1] 

机构地区：[1]辽宁中医药大学,沈阳110032

出　　处：《世界中医药》2013年第1期71-73,共3页World Chinese Medicine

摘　　要：目的:观察气滞血瘀证大鼠一氧化氮/一氧化氮合成酶(NO/NOS)的体系变化。方法:将Wistar大鼠随机分成4组,对照组、模型组、血府逐瘀汤低、高剂量组。采用多因素联合刺激法建立气滞血瘀模型。运用分光光度法测定血浆和肝组织中NO含量以及肝组织中NOS活性,利用RT-PCR法测定肝组织中NOS基因表达。结果:模型组血浆NO含量(0.52±0.33)μmol/L显著低于对照组(2.77±1.73)μmol/L(P<0.01),低剂量组NO含量(2.37±0.53)μmol/L显著高于模型组(0.52±0.33)μmol/L(P<0.01);模型组肝组织NO含量(0.52±0.24)μmol/mg显著低于对照组(5.87±1.72)μmol/mg(P<0.01),低剂量组NO含量(3.72±1.53)μmol/mg显著高于模型组(0.52±0.24)μmol/mg(P<0.01);模型组肝组织eNOS基因表达水平显著低于对照组(P<0.01),低剂量组肝组织eNOS显著高于模型组(P<0.05)。结论:气滞血瘀证大鼠NO/NOS体系下调,为进一步研究治疗血瘀症类疾病提供了借鉴和思路。Objective： To investigate change of nitric oxide （NO）/NO synthase （NOS） in Qi-blood stagnation of rats. Methods ： Wistar rats were randomized to four groups： normal control group, model group, low concentration group, and high concentration group. The Qi-blood stagnation model was established by complex pathogens. The content of NO in plasma and liver tissues of rats was measured by spectrophotometric. RT-PCR was used to analyze liver tissues NOS mRNA expression. Results： The plasma NO of model group [ （0.52±0.33）μmol/L] decreased significantly compared with normal control group[ （2.77±1.73）μmol/L] （P 〈0.01 ）. The plasma NO of treatment group [ （ 2.37±0.53 ）μmol/L ] increased significantly compared with model group [ （ 0.52±0.33 ） μmol/L ] （ P 〈 0.01 ）. The NO in liver tissues of model group [ （0.52±0.24 ） μmol/mg ] decreased significantly compared with normal control group [ （5.87 ± 1. 72 ） μmol/mg ] （ P 〈 0.01 ）. The NO in liver tissues of treatment group [ （ 3.72 ±1.53 ） μmol/mg ] increased significantly compared with model group[ （0.52±0.24） μmoL/mg] （P 〈0.01 ）. The expression of NOS in liver tissues NOS mRNA expression in model group de-creased significantly compared with normal control group （P 〈 0.01 ）. The expression of NOS in liver tissues NOS mRNA expression in low concentration increased significantly compared with model group （P 〈0.05 ）. Conclusion： Nitric oxide（ NO）/NO synthase（NOS） was down-regulated in Qi-blood stagnation rats.

关 键 词：气滞血瘀 一氧化氮 一氧化氮合成酶 

分 类 号：R-332[医药卫生]