李与杏种间杂种的RAPD和SSR分析  

Identification of Hybrids between Prunus salicina and Armeniaca vulgaris by RAPD and SSR

作　　者：杨红花[1] 秦宏伟[2] 米玛潘多 肖军[1] 

机构地区：[1]泰山学院生物与酿酒工程学院,山东泰安271021 [2]泰山学院机械与工程学院,山东泰安271021 [3]西藏自治区高原生物研究所,西藏拉萨850001

出　　处：《安徽农业科学》2013年第2期497-498,605,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省自然科学基金(Q2008D01);山东省高校科研发展计划项目(J09LC54)

摘　　要：[目的]探讨杂种苗与亲代之间的遗传变异及其真伪性,获取快速鉴定杂种的方法。[方法]利用RAPD和SSR分子标记技术对凯特杏和莫尔特尼李的种间杂交种进行分析。[结果]叶片形态、RAPD和SSR技术对杂种的鉴定分析,表明子代与亲代电泳图谱之间存在差异,所获杂种为真杂种。[结论]将RAPD及SSR技术结合可有效鉴定李杏种间杂交种,是一种快速鉴定杂种的方法,可缩短育种周期,提高育种效率。[Objective] This study aimed to investigate the genetic variation between parents and hybrids,and estimate they were true or not,to find a rapid identification method.[Method] The progenies between Armeniaca vulgaris Katy and Prunus salicina Moriteni were analyzed by means of RAPD and SSR molecular markers.[Result] Electrophoresis differences between progenies and parents were observed,indicating that hybrid seedlings obtained were real.[Conclusion] The combination of the above method is effective and fast to identify the hybrids,and can shorten the breeding period.

关 键 词：李 杏 种间杂交 RAPD SSR 

分 类 号：S662.3[农业科学—果树学]