小麦中呕吐毒素的ELISA和HPLC法检测  

ELISA and HPLC for Detection of DON in Wheat

作　　者：杨丹妮[1] 汪海峰[2] 

机构地区：[1]江苏省常州市产品质量监督检验所,江苏常州213100 [2]南京财经大学,江苏南京210009

出　　处：《安徽农业科学》2013年第3期1269-1270,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]考察自主研制的呕吐毒素ELISA试剂盒检测结果的准确性。[方法]对小麦样品进行加标回收试验,样品经提取后直接过滤即可用于ELISA检测,过滤后再过免疫亲和柱的样液同时经HPLC和ELISA检测。[结果]提取后的样品直接过滤后进行ELISA检测的回收率较高,在75%以上,相对标准偏差(RSD)在4.7%～10.6%;而过柱后经HPLC法和ELISA法检测,在加标浓度较高的情况下,两者回收率相当,分别为49.8%和49.7%,相对标准偏差(RSD)分别为1.8%和5.6%。[结论]采用呕吐毒素试剂盒检测小麦样品的结果准确可靠,方法简便快捷,适合大量样品的快速筛选。[ Objective ] To investigate the accurate of ELISA detection result. [ Method] Test for recovery was made by the standard addition method, wheat samples were extracted and filtered, followed by ELISA. The filtrate was purified by inununoaffinity column and analyzed by I-IPLC and ELISA. [ Result] The results showed that the recovery of the direct filtrate analyzed by ELISA was more than 75%, and RSD was in the range of 4.7% - 10.6%. Recoveries of the filtrate being purified were 49.8% and 49.7% respectively, and RSD was 1.8% and 5.6% respectlvely, and there was no significant difference between them at high concentrations of added standard solutions. [Conclusion] The ELISA kit for detection of DON is simple, rapid, accurate and is suitable for the rapid screening of large number of samples.

关 键 词：小麦 呕吐毒素 酶联免疫法 高效液相色谱法 检测 

分 类 号：S512.1[农业科学—作物学]