2种方法提取柑橘皮中挥发性成分的气质联用分析  

Comparing Two Kinds of Methods for Extraction of Volatile Oils from Orange Peel and Components Analysis by GC-MS

作　　者：薛成虎[1] 王瑞斌[1] 

机构地区：[1]榆林学院化学与化工学院,陕西榆林719000

出　　处：《安徽农业科学》2013年第3期1283-1285,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]分析比较不同方法提取柑橘皮中挥发性成分的效果。[方法]分别采用水蒸气蒸馏(SD)和超声萃取-水蒸气蒸馏(UESD)从柑橘皮中提取挥发油,通过气相色谱-质谱(GC-MS)联用技术,比较分析了挥发油的化学成分;峰面积归一化法确定了各组分的相对百分含量。[结果]SD法共分离出30个色谱峰,鉴定出21种物质,占总峰面积的93.89%,主要是邻苯二甲酸(51.93%);UESD法共分离出45个色谱峰,鉴定出38种物质,占总峰面积的98.07%,主要是D-苎烯(25.90%)等。[结论]2种方法提取柑橘皮中挥发油主要共有成分为有机酸和烷烃类化合物,但组成和含量有差异,SD法提取有机酸的含量较高。[ Objective] To compare different extraction method for volatile oil from orange peel. [ Method] The volatile oils were extracted from orange peel by steam distillation （SD） and ultrasound-assisted steam distillation （UESD）, respectively. The chemical constituents in the volatile oils were identified by Gas Chromatography-Mass Spectrometry GC-MS and their relative contents were calculated based on GC peak ar- eas. [ Result] For steam distillation, 21 compounds of 30 detected peaks were identified, accounting for 93.89% of the volatile fraction. The main components were dimethyl phthalate（51.93% ） ; For ultrasound-assisted steam distillation, 38 compounds of 45 detected peaks were i- dentified, accounting for 98.07% of the volatile fraction. The main components were D-Limonene. [ Conclusion] The results indicated that, there is a significant difference in the composition and relative amounts between the volatile oils obtained by SD and UESD methods; the com- mon components of the two extracts were organic acids and alkanes. SD method allows higher amount of organic acids than UESD.

关 键 词：柑橘皮 挥发油 GC-MS 水蒸气蒸馏 超声萃取-水蒸气蒸馏 

分 类 号：S666[农业科学—果树学]